Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification

1、

METHODS the K-B manner and MIC are used to obtain drug-fast spectrum and the alkaline lysis is to extract small quantity of plasmid is got from cataphoresis of 0.7% agarose congelation.

方法耐药谱选用K&B法和MIC、Alkaline Lysis小量快速提取质粒，0.7%琼脂糖凝胶电泳获得质粒DNA图谱。

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2、

[ Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability.

方法使用替代试剂，建立改良的碱裂解法进行质粒DNA提取，并用琼脂糖电泳验证所得的质粒的质量，紫外测量估算产量及纯度。限制性酶切验证其用度。

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3、

The plasmid DNA was extracted by Alkaline Lysis, and separated by electrophoresis on the gel.

Alkaline Lysis小量快速提取质粒DNA，凝胶电泳拍照；

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4、

In conclusion, sufficient DNA for PCR can be quickly extracted from transgenic rice food by alkaline lysis method. The stability of recombinant DNA in transgenic was different under different processing conditions.

由此可知，碱处理法能够快速、有效的提取转基因稻米制品中DNA，转基因稻米中的各种外源成分在不同加工条件下的稳定性不同。

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5、

An improved alkaline lysis adaptive to extracting plasmid DNA of Bacillus

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

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6、

Methods: Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates, and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains.

方法：采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性；碱裂解法分析产青霉素酶的淋病奈瑟菌（PPNG）株及耐四环素的淋病奈瑟菌（TRNG）株的质粒图谱；

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7、

Samples were extracted with the 80% precooling and acidified methanol and conjugated abscisic acid in extracts were treated by alkaline lysis method.

样品以80%酸化预冷甲醇提取，使用碱裂解法分析检测ABA结合态。

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8、

Conclusion The alkaline lysis method is simple, rapid and efficient for extraction of serum HBV-DNA and suitable for HBV-DNA quantitative PCR detection.

结论碱裂解法提取HBV-DNA简单、快速，适用于HBV-DNA荧光定量测定。

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9、

New Application Technology of Chemical Nickel phosphorus Plating from alkaline bath at Middle Temperature

中温碱性化学镀镍磷实用新工艺研究

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10、

Copper Electroplating in Alkaline Bath Containing Novel Brightening Agent

采用新型组合光亮剂的碱性镀铜研究

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11、

And the coating obtained in alkaline bath is of crystal structure, belonging to low phosphorous coating.

在碱性镀液中获得的镀层是晶态的，属于低磷镀层。

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12、

Selection of Formulation of Combined Additives for Electroplating of Zinc-Nickel Alloy Coating from an Alkaline Bath

碱性体系中锌镍合金电镀添加剂复合配方优选

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13、

Studies on white chromating of zinc-nickel alloy electrodeposited from alkaline bath

碱性锌镍合金电镀白钝化工艺的研究

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14、

Electrodeposition of Zn-Ni Alloy in alkaline bath

碱性锌镍合金电镀

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15、

Further more, the ratio of the main compositions of the alkaline bath is very important for its tempering effect. The results strongly indicate that the contents of alkali and the anti-oxidation reagent can significantly affect the hardness.

碱浴的配比很重要，其中碱和脱氧剂的用量对回火硬度影响显著。

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16、

The zinc nickel alloy depositions with grain size of 14-33 nm were electroplated by using adequate additives in an alkaline bath.

采用适当的添加剂在碱性体系中获得了晶粒度为14&33nm的纳米锌镍合金电镀层。

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17、

The changes of surface potential and topography of Fe-Mo amorphous alloy coatings deposited In original alkaline bath at room temperature were investigated.

研究了非晶态Fe-Mo合金镀层在室温下的原碱性镀液中或其他溶液介质中的表面电位、表面形貌的变化。

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18、

Transparent and thin copper layer in various colours can be electroplated onto stainless steel surfaces from an alkaline bath with low current density.

在碱性铜盐溶液中以低电流密度对不锈铜进行电镀可获得多种彩色鲜艳的透明薄铜层。

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19、

A Study on Dyeing Property of Superfine Fiber with Disperse Dye in Alkaline Bath

超细纤维织物国产分散染料碱性浴染色性能研究

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20、

The Technology and Properties of Superfine Polyester Fabric Dyed in Alkaline Bath

超细涤纶织物碱性浴染色工艺研究

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