We obtained the auxotrophic mutantS of k ( lys, p) and Q ( arg-) by mutation with DES. The frequency of fusion is 1. 25 per 105 protoplast.

1、

The effect of volumetric oxygen transfer coefficient ( kLa), on the performance of producing pyruvate by a multi-vitamin-auxotrophic yeast of Torulopsis glabrata, was investigated in batch fermentation process.

以一株光滑球拟酵母的多重维生素营养缺陷型为研究菌株，考察了分批发酵中不同体积传氧系数（KLa）对其产丙酮酸性能的影响。

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2、

By using microcalorimetry method, we have studied ura auxotrophic mutant of saccharomyces cerevisiae AY, and 3 strains transformed from saccharomyces cerevisiae AY by plasmids.

用微量热法研究了尿嘧啶缺陷型酿酒酵母AY，和该菌株分别经穿梭质粒pYLZ-2、重组质粒pYLZ-2/f27、pYLZ-2/622转化的3种菌株的生长代谢热动力学。

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3、

Then at the 6 point will lead to a higher pregnancy rate. The reverse mutation frequency of the protoplast of the auxotrophic mutants was less than 10 ￣(-6).

营养缺陷型菌株原生质体回复突变率小于10￣（－6）。

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4、

Further investigation demonstrated that UV light decreased the mating ability of ade auxotrophic haploids and had no significant effect on the mating ability of other auxotrophic haploids.

研究表明：UV减弱了腺嘌呤缺陷型单倍体的交配能力，而对其它营养缺陷型单倍体的交配能力无甚影响；

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5、

Auxotrophic mutants of Trichoderma harzianum tolerant to carbendazim were obtained by UV-light mutagenesis and carbendazim induction on PDA medium.

用紫外线诱变哈茨木霉野生型菌株，经含多菌灵PDA培养基耐药性筛选，获得了耐药性菌株。

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6、

Prototrophic hybrids have been obtained by the protoplast fusion between auxotrophic haploid and diploid mutants of Saccharomyces cerevisiae.

本文采用营养缺陷型的互补进行了啤酒酵母原生质体融合产物的筛选。

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7、

Mutagenesis of Auxotrophic Mutants of Penicillium chrysogenum by NTG

NTG诱发产黄青霉菌产生营养缺陷型突变体的研究报道

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8、

Induction and selection of auxotrophic mutants from the protoplasts of Lentinus edodes screening and characteristics of mutants of E. coli resistant to acetate inhibition

香菇原生质体诱变及营养缺陷型突变体筛选大肠杆菌乙酸代谢突变株的选育和特性研究

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9、

Nine auxotrophic mutant strains were obtained by using~ ( 60) Co-γ ray irradiating Auricularia basidiospores.

利用~（60）Co&γ射线辐照木耳担孢子，获得了9株营养缺陷型突变体。

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10、

The optimal conditions for protoplast isolation and regeneration of4 auxotrophic mutant strains of Trichoderma were studied.

研究了4种营养缺陷型产纤维素酶菌的原生质体形成和再生条件。

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11、

Selection of high α-amylase yeilding strains of Bacillus subtilis by auxotrophic reversion technique

应用营养缺陷型回复突变的方法筛选枯草杆菌（Bacillus subtilis）α-淀粉酶的高产菌株

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12、

Auxotrophic Mutant Selection and Protoplast Fusion in Saccharomyces cerevisiae

啤酒酵母（saccharomyces cerevisiae）营养缺陷型的筛选及原生质体融合

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13、

Preparation of double mutants through crosses between compatible auxotrophic mutants in higher basidiomycetes

高等担子菌双重营养缺陷突变菌株的分离

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14、

Studies on the Regeneration Mode of the Protoplast of Auxotrophic Mutants of Auricularia

木耳营养缺陷型原生质体再生方式研究

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15、

Selection of the Optimum Conditions of Preparing an Auxotrophic Saccharomyces cerevisiae Protoplast by Orthogonal Tests

用正交实验法筛选营养缺陷型酿酒酵母原生质体的最佳实验条件

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16、

Mutagenic Effects of Laser on Auricularia auricula and Selection of Auxotrophic Mutant

黑木耳激光诱变及营养缺陷型突变体筛选

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17、

Analysis of Availability Index of Quantitative Forecast Method

定量预测方法有效性指标分析

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18、

Subjective and objective support and support availability have negative forecast to loneliness.

主观支持、客观支持和支持利用度对孤独感总分及各个维度具有一定的负向预测作用。

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19、

No technological and therapeutical improvements can be practically utilized if the material means for its utilization have not been previously made available by saving and capital accumulation.

改进了工艺学与治疗学，如果没有储蓄与资本累积预先准备了物质条件，也就无法实际利用了。

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20、

Nevertheless, the rapid development is a hindrance to the available running of the capital market in china. The optimization of market structure is one of these problems that are notable.

然而，过快的发展速度也为资本市场的健康有效运行埋下了隐患，资本市场结构优化问题就是其中显著的问题之一。

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