以基因组DNA为模板,分别加入针对K-ras第12密码子的三种主要突变方式设计的PCR上游引物R1、R2、R3和共同的下游引物R4,在taq DNA聚合酶作用下扩增相应片段。
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方法:14例青春期及儿童GCT选自1957~1995年我科收治的216例GCT病人,随访2~12年,总结其临床表现,放射学特点,治疗及随访结果。
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表面活性剂以“奇强”洗洁精对提高纤维素酶活力作用最显著,用量为0.5%时酶活力比对照提高12.4%。
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Method: We observed the change of respiratory before and after secondary thoracotomy of 12 patients.
方法: 观察12例二次开胸手术病人手术前后呼吸功能变化.
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方法: 12例高位食管癌采用非开胸食管内翻拔脱术.
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Police closed off about 12 blocks of a major San Francisco thoroughfare for today's march.
由于今天的示威游行,警方封锁了旧金山一条主干道上大约12个街区。
《柯林斯高阶英汉双解学习词典》
The incidence rate of adverse effect in irbesartan group was 12 . 5 %, and benazepril group 22.58 %.
不良反应发生率国产伊贝沙坦组为12.5%, 苯那普利组为22.58%(p& gt;0.05).
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Its titer was 1:12 000 . Conclusion : The production and purity of IgY were high.
ELISA测定抗白念珠菌IgY抗体的效价达到1∶12000.
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In addition, the torpedo system filled with water within 12 minutes filled with 6 torpedoes!
此外, 它的鱼雷水压装填系统可在12分钟内装填6枚鱼雷!
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Results 16 eyes had tractional detachment of retina of retina 12 eyes had vitreous opacity.
结果28眼巩膜裂伤中出现牵拉性网脱16眼、单纯性玻璃体浑浊12眼.
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A strategist at a local trade association predicts that " 2011-12 will be carnage year. "
在当地行业协会的战略家预言道: “ 2011-12年度 将是的屠杀之年. ”
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Vegans consume no animal products and are susceptible to vitamin B 12 deficiency.
素食者不吃任何动物性食物,易患维生素B12缺乏症.
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The subscription of shares since the end of the issue date of 12 months transferable.
认购的股份自发行结束之日起12个月内不得转让.
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In Iran, gunmen posing as police officers waylaid four cars and killed 12 passengers.
伊朗, 8名持枪歹徒打扮成警察截住4辆汽车,枪杀了车上的12名乘客.
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Nominal cooling capacity conditions : chilled water in 12℃, out 7℃; entering air DB 35℃ , WB 24℃.
冷量 系依据冰水入口温度12℃, 出口温度7℃; 入口空气干球温度35℃, 湿球温度24℃计算得出.
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