I stepped on the bus and looked down the aisle, all I saw were a bunch of people with bus looks minding their own business.

Do you think we'll ever get to meet an alien from outer space?

你觉得咱们会在外太空遇到外星人吗？

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Alien from a single network rumor, the object we discussed in this paper is the network rumor which arises from emergency.

与单一网络谣言不同的是，突发事件中的网络谣言，是依附于突发事件这一载体而产生的。

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I am an alien from another planet.

我是一位来自其他星球的外星人。

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For the influence of various factors, American historiography after World War II tended to be alien from the grand narrative.

由于各种因素的影响，二战后的美国史学出现疏离宏大叙事的趋势。

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Its duality makes drama translation alien from translations of other literary styles.

这一独特性决定了戏剧翻译与其他文体的翻译有所不同，对于翻译者来说，戏剧翻译更具挑战性。

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This style is alien from genuine English.

这种文体与纯正的英语不同。

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What's not good for my baby is sharing my home with a killer alien from outer space.

和外星杀手共处一室才是对宝宝不好

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If you were an alien from Mars and sat in on one of these meetings, you would believe that a clients success or progress was not part of the thought process at all.

如果您是一位来自火星的外星人并且参与到其中的一个会议，你会感觉到，客户的成功和进步完全不是会议的议题。

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It had an effect entirely alien from the one intended.

其结果兴所预期的完全相反。

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Why not just tell people I'm an alien from Mars.

为什么不直接告诉人们我就是个来自火星的外星人呢？

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Finally, alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

最后用碱裂解法裂解细胞提取质粒，并将提取物进行阴离子交换层析以进一步纯化质粒。

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The final yield of plasmid DNA from the broth after alkaline lysis is 56.61%, and its purity determined by agarose electrophoresis is almost 100%.

结果表明：以碱裂液中质粒DNA为计算基准的得率为56.61%、超螺旋质粒DNA的电泳纯度100%。

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The procedure of Southern hybridization has been improved as follows: the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation.

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

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The plasmids were extracted by alkaline lysis technique.

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

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Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

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Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

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After such pre-treatments, the materials were denatured by a modified alkaline lysis procedure and their mtDNA was extracted.

经过处理后的材料分别再用改良的碱变性法[2～6]提取mtDNA，并比较相应处理的mtDNA。

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Thirty genomic DNA of Fusarium were extracted with improved SDS alkaline lysis method and the ITS district of rDNA was especially amplified.

实验运用改良的SDS碱裂解法对30株镰刀菌基因组DNA进行了抽提，并对rDNA ITS区域进行了特异性扩增。

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Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA.

目的对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

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Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay.

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；

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