Application value of FQ-PCR for detection of treponema pallidum DNA

Objective To evaluate an enzyme-linked immunosorbent assay ( ELISA) technique based on recombinant antigens for detection of antibodies against Treponema pallidum.

目的评价基因重组抗原ELISA法在梅毒螺旋体抗体检测中的意义。

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The 4 rapid tests and TPHA were performed by using whole blood and serum respectively.

分别取全血和血清进行梅毒螺旋体血凝试验（TPHA）及4种试剂盒的检测。

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Objective To approach double-primer PCR assay for simultaneous detection of Herpes simplex virus ⅱ( HSV-2) and Treponema pallidum.

目的建立双引物聚合酶链反应（PCR）用于同时快速检测梅毒螺旋体和单纯疱疹病毒Ⅱ型。

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Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity, and hence can be applied in the diagnosis of syphilis in various periods.

结论梅毒螺旋体特异性IgM抗体检测灵敏度高、特异性强，适用于未经治疗的各个时期的梅毒诊断。

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Clinical evaluation of specific IgM treponema pallidum antibody detection

梅毒螺旋体特异性IgM抗体检测的临床评价

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An optimized method has been established to simultaneously detect multiple antibodies of HIV, TP and HCV in the serum by the way of microarray.

建立一种用蛋白质微阵列法可同时检测血清中艾滋病毒（HIV），丙型肝炎病毒（HCV）和梅毒螺旋体（TP）抗体的方法。

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51 clinical samples were detected by dark-field microscopy and a double-primer PCR, the positive rates were 15.7% and 19.6%, respectively.

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

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Diagnostic Significance of IgM Antibodies to Treponema Pallidum in Cerebrospinal Fluid for Neurosyphilis

梅毒螺旋体IgM抗体对于神经梅毒的诊断意义

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Controlled experiments of testing anti-TP and anti-HIV by DIGFA and EIA

免疫胶体金与酶免疫检测HIV抗体和梅毒螺旋体抗体对照实验

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Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods.

目的探讨梅毒螺旋体特异性IgM抗体检测在各个时期梅毒诊断中的意义。

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Methods 2557 cases were detected by using ELISA with double antigen sandwich before blood transfusion.

方法：采用免疫ELISA法检测2557例输血前患者血清梅毒螺旋体抗体。

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Objective: To establish PCR assay for rapid detection of Treponema pallidum.

目的：建立聚合酶链反应（PCR）用于快速检测梅毒螺旋体。

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Conclusions In view of its high specificity and sensitivity, TP-IgM-WB could be used as a confirmatory test for the diagnosis of both symptomatic and asymptomatic neonatal congenital syphilis.

结论梅毒螺旋体IgM抗体蛋白印迹试验可作为一种确诊试验应用于新生儿胎传梅毒，尤其是胎传潜伏梅毒的早期诊断。

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Methods: According to specific sequence of Treponema pallidum gene ( tpp47 gene), a pair of specific primers were designed and PCR assay was developed.

方法：根据梅毒螺旋体47ku膜蛋白基因序列，自行设计筛选一对特异寡核苷酸引物，建立梅毒螺旋体的PCR。

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ELISA with Double Antigen Sandwich for Screening Speeific Serum Anti-TP Antibody in Blood Donors

应用双抗原夹心ELISA法筛查献血员梅毒螺旋体抗体

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Construction of fusion bivalent DNA vaccine of Treponema pallidum and its immune activity

梅毒螺旋体融合双价DNA疫苗的构建及其免疫活性研究

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Objective To understand the infection of TP among HIV carriers in Yunnan province, and to offer scientific bases for surveillance STD.

目的了解HIV感染者中梅毒螺旋体（TP）在我省的感染状况，为监测性传播疾病（STD）提供科学依据。

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Objective To investigate the rates of false-positive reactions to Treponema Pallidum ( TP) of patients with malignant tumors and to analyze their clinical features.

目的了解恶性肿瘤患者梅毒螺旋体（Treponema pallidum，TP）抗体假阳性率并对阳性患者进行临床分析。

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Amplification of the DNA Polymerase I Gene of Treponema Pallidum by PCR and its Application in Diagnosis of Primary Syphilis

PCR扩增梅毒螺旋体poIA基因及其在一期梅毒诊断中的应用

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Passive particle agglutination test for detection of TP AB ( TPPA);

梅毒螺旋体特异性抗体明胶凝集试验（TPPA）；

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