[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated.

1、

One of the mechanisms causing gene mutation might be to change of the Taq DNA polymerase conformation. It interfers with the enzyme-substrate-dNTP binding site and further causes replication mismatch.

这一实验结果也说明了稀土是一种诱变剂，引起基因突变的机制之一可能是改变taq DNA聚合酶的立体构象，干扰了酶与底物dNTP结合位点，从而引起复制错配。

互联网摘选

2、

The total DNAs from 3 species of plants and 2 species of animals were amplified by RAPD ( random amplified polymorphic DNA) using 10 RAPD 10 nt primers and 3 different commercial Taq DNA polymerases, respectively.

随机选用了10条RAPD引物，采用3种商品taq DNA聚合酶体系，以5个不同物种的动植物总DNA为模板，进行RAPD扩增。

互联网摘选

3、

High content of protein, phenol, tannin, pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响taq DNA聚合酶的活性，是导致PCR扩增反应失败的主要因素。

互联网摘选

4、

Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR.

在等位基因特异PCR中，Mg2+、dNTP及taq DNA聚合酶的用量均大于普通PCR。

互联网摘选

5、

Taq DNA polymerase is an ideal enzyme for DNA sequencing because it's fast, highly processive and active over a broad range of temperature.

taq DNA聚合酶具有反应速度快、温度作用范围广及良好的续进性等特点，可视为一种理想的DNA顺序分析酶。

互联网摘选

6、

This study concentrated on extracting and purification of Taq and Pfu DNA polymerase in the recombinant strains.

本文主要研究从重组工程菌中分别提取Pfu DNA聚合酶及taq DNA聚合酶。

互联网摘选

7、

Optimization of Preparation Techniques of Taq DNA Polymerase

taq DNA聚合酶制备技术的优化

互联网摘选

8、

Study on the Functional Domain of Taq DNA Polymerase

taq DNA聚合酶功能区域的定位

互联网摘选

9、

Optimization of Recombinant Taq DNA Polymerase Expression in E.coli

重组taq DNA聚合酶在大肠杆菌中表达条件的优化

互联网摘选

10、

Direct Amplification of Target Sequences by Taq DNA Polymerise Using Double-stranded Rna as Templates

利用taq DNA聚合酶直接从双链RNA模板中扩增靶序列

互联网摘选

11、

General Taq DNA polymerase amplified long DNA fragments of Helicobacter pylori urease

普通taq DNA聚合酶扩增幽门螺杆菌尿素酶基因长片段

互联网摘选

12、

Separation and Purification of Taq DNA Polymerase in E. coli

大肠杆菌表达重组taq DNA聚合酶的分离和纯化

互联网摘选

13、

Rapid Purification of High-activity Recombinant Taq DNA Polymerase Using Freezing-thawing Method

以冻融法快速纯化高活力基因工程taq DNA聚合酶

互联网摘选

14、

Direct DNA Sequencing of Polymerase Chain Reaction Products With Taq DNA Polymerase

利用taq DNA聚合酶对PCR产物直接进行顺序分析

互联网摘选

15、

I'll tell you these two nights we have been out here give me the creeps.

不瞒你说,我们在这儿守了两夜,我是夜夜胆战心惊.

词典精选例句

16、

Ivy creeps along the fence.

常春藤攀着篱笆生长.

词典精选例句

17、

The night creeps slowly on.

夜色慢慢来临.

词典精选例句

18、

The grape-vine creeps along the wall.

葡萄藤沿墙蔓延.

词典精选例句

19、

Age creeps upon him; nevertheless, he works as hard as ever.

他不知不觉地老起来了; 可是他照常努力工作.

词典精选例句

20、

I don't like him: he gives me the creeps.

我不喜欢他,他这人很讨厌.

词典精选例句