The Adaptation of Rabies Virus, Strains in Vero Cell Morphological study for the different strains of fixed rabies virus

Morphological study for the different strains of fixed rabies virus

狂犬病病毒不同毒株的形态学研究

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Wuhan-34 strain of rabies virus was successfully propagated in primary chick embryo cell culture. It's immunogenicity has shown that it can compare with that of the fixed virus aG strain used for hamster kidney cell rabies vaccines production in our country.

初步成功地培植出一株适应原代鸡胚细胞，具有与国内目前生产地鼠肾细胞疫苗的aG株固定毒相仿的免疫原性的组织培养疫苗株&武汉（Wuhan）34株。

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Objective To analyze the sequence of glycoprotein ( GP) gene of three passages of rabies vaccine strain CTN-1-V in China and compare with that of street virus strain.

目的分析三个代次CTN-1-V病毒株糖蛋白（GP）基因部分序列，并与代表性街毒株进行比较。

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Comparison of G Genes of Novel Rabies Street Virus Strains Isolated in Zhejiang Province, China and Other Vaccine Virus Strains

浙江新分离狂犬病病毒街毒株与国内外疫苗株G基因的比较分析

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[ Abstract] Objective To study on the immunological characteristics of 4 rabies street virus strains isolated in China.

目的研究国内分离的4株狂犬病街毒的免疫学特性。

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Application of McAbs against rabies nucleocapsid in diagnosis of rabies street virus

抗狂犬病毒核蛋白单克隆抗体在狂犬病街毒检测中的应用

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Conclusion aG vaccine showed good cross immunity to the 4 street virus strains.

结论aG疫苗与4株街毒具有良好的交叉免疫性。

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Gene variation was observed between the street virus strain and vaccine strain.

街毒与疫苗株之间基因存在差异。

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Rapid Detection of Rabies Street Virus Antigen

狂犬病毒街毒抗原的快速检测

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Variola virus belongs to the genus of orthopoxvirus, and there are some other infectious members, such as Vaccinia virus, cowpox virus, and monkeypox virus.

在正痘病毒属还有另外几种病毒对人具有一定的感染性，如痘苗病毒、牛痘病毒和猴痘病毒。

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It belongs to a group of viruses that includes the smallpox virus ( variola), the virus used in the smallpox vaccine ( vaccinia), and the cowpox virus.

它属于一个包括天花病毒、在天花疫苗中采用的病毒和牛痘病毒的病毒组。

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DNAs from Cowpox virus are also used and experiments found that hybridization signals were strong, which suggested that the oligo-microarray could be used in detection of orthopoxvirus.

标记的牛痘病毒DNA与芯片杂交也有明显的杂交信号，进一步验证了该芯片用于正痘科病毒检测的可行性。

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A silent nucleotide change was introduced into the cDNA clone to create a Xba I site ( C to T at 8453nt) that is used as a genetic marker of this infectious clone. This genetic marker was retained in the genome of recovered progeny virus.

全基因组cDNA克隆构建过程中引入的沉默突变（8453位核苷酸由C变为T）产生Xba I酶切位点作为遗传标记，在子代恢复病毒的基因组中稳定存在。

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Beginning in1991, my colleagues and I had the privilege of working with structural biologists and medicinal chemists to test small chemicals that might intercalate into the catalytic site of the HIV protease, an enzyme essential for the production of infectious progeny virus.

从1991年开始，我的同事和我有幸与结构生物学家和医学化学家合作，对有可能阻隔艾滋病毒蛋白酶的触媒点的微型化学药物进行测试，这种蛋白酶是繁殖传染性病毒的必要酵素。

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Can inhibit replication of DENV and WNV, RIG-I was transiently over-expressed in A549 cells followed by infection with DENV or WNV, and the progeny virus yield decreased sharply in a dose-dependent manner.

我们进一步通过过表达的方法观察了RIG-I对登革病毒和西尼罗病毒复制的抑制效果，结果发现RIG-I在A549细胞中的过表达能够显著降低子代病毒的产量，并呈剂量依赖性。

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Results: Most of the progeny virus plaques ( 70.83% of 24) had the parental genotype of 76-118 strain or R22 strain.

结果：发现24株子代病毒（70.83%）的基因组来自76-118株或R22株；

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There was no significant antiviral activity to be detected when the GLP was presented in the culture after beginning infection and before progeny virus release.

如果在病毒感染细胞后加入GLP，在病毒的生物大分子的合成完成后而子代病毒粒子还未释放出来前去掉GLP，这时GLP对病毒感染就没有抑制作用。

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Infection of susceptible cells with a virus in many cases results in the multiplication of progeny virus.

多数情况下，易感细胞感染病毒后，可导致子代病毒的增殖。

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Amino acid substitutions were present in progeny virus by RT-PCR.

RT-PCR表明引入的氨基酸突变在子代病毒中存在。

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However, the mechanism of secondary bacterial infection following virus infection is still unknown.

然而，呼吸道病毒感染继发细菌感染的机制至今尚不清楚。

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