After PCR and sequencing, the expression plasmid pWD κ H was transfected into CHO ( dhfr ~-) cells.
经PCR和测序鉴定正确后, 用lipofectAMINE2 000转染CHO ( dhfr - ) 细胞.
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Construction of targeted integration and high expression system in CHO/ dhfr-cell line
新型CHO/dhfr~-细胞定点整合和表达系统的建立
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Analysis and expression in CHO/ dhfr~+ of recombinant human protein C
重组人蛋白C在CHO/dhfr~+细胞中的表达与分析
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The products of TS gene and DHFR gene were 403 and 273 bp respectively.
结果卡氏肺孢子虫TS基因和DHFR基因的扩增产物分别为403和273bp。
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