1、

Two pair of primers containing attB adapter were designed separately by Gateway cloning technology. Platinum pfx DNA polymerase which can reduce the non-specific binding greatly was used during two PCR in which adding B sequence to the cloned gene.

根据Gateway克隆技术的要求,设计含有attB接头的引物,利用高保真的PlatinumpfxDNA聚合酶,通过PCR方法在克隆基因的两端加上B序列。

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2、

Objective: The experiment was designed to research the relationship between mitochondrial DNA ( mtDNA) mutation and aminoglycoside antibiotic induced deafness ( AAID) and establish a gene diagnostic method.

目的:研究线粒体DNA突变与氨基糖甙类抗生素致聋(AAID)的关系,建立相应的基因诊断方法。

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3、

Correlation between DNA Repair Gene XPD Polymorphism and p53 Mutation

非小细胞肺癌患者DNA修复基因XPD多态性与p53基因突变的相关性研究

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4、

Objective To study the association between polymorphism of DNA repair gene xeroderma pigmentosum G ( XPG) Asp1104His and the risks of developing laryngeal and hypopharyngeal carcinomas.

目的研究DNA修复基因着色性干皮病G组基因(XPG)Asp1104His多态性与喉癌和喉咽癌风险的相关性。

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5、

Polymerase chain reaction-PCR technique was used to obtain Vitreoscilla hemoglobin gene ( vgb) using its genomic DNA as a template.

以透明颤菌染色体基因组DNA为模板,利用PCR技术获取透明颤菌(Vitreoscilla)血红蛋白基因(vgb)。

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6、

VBL can help verifying DNA computing theoretic model and improving biological experiment efficiency.

在实际应用中,VBL可以帮助验证DNA计算的理论模型,提高生物实验效率。

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7、

Raman Spectroscopic Analysis of Influence on Herring Sperm DNA with UVA& UVS Radiation

UVA和UVB区段紫外线照射对DNA影响的拉曼光谱分析

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8、

Objective: To express and purify TDG protein, and to prepare the rabbit antibody against thymine DNA glycosylase ( TDG) protein.

目的:表达纯化胸腺嘧啶糖苷酶(TDG)蛋白并制备TDG多克隆抗体。

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9、

The Government could simply copy the existing ACPO Guidelines, which make it as hard as possible for innocent people to have their DNA profiles and samples and fingerprints, removed.

政府可以照搬现有的ACPO准则,这使得像可能无辜的人有他们的DNA图谱和样品和指纹,删除。

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10、

Genome DNA was extracted by TKM method. Exon 10 of TSHR gene sequence was amplified, PCR production was detected by direct and reverse sequencing, and the results and human TSHR gene sequence Genbank were compared.

用TKM法提取基因组DNA,PCR扩增TSHR基因第10外显子序列,对PCR产物正反向测序,测序结果同Genbank人TSHR基因序列对比。

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11、

In 42 cases recovery model group ( RMG) samples, 4 cases were HBV DNA positive with a positive rate of 9 52%.

恢复期模式组有42例,但仍可检出HBV dna4例,阳性率为9.52%;

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12、

PMX caused DNA damages in murine embryonic stem cells, oxidative stress injuries of mice and apoptosis of embryonic stem cells, leading to abnormal closure of neural tube and ultimately NTDs.

体外实验中,PMX可以损伤小鼠胚胎干细胞DNA,造成小鼠氧化应激损伤,使胚胎干细胞发生凋亡,最终使神经管不能发生正常闭合,而导致神经管畸形的发生。

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13、

Objective To establish an in vitro model of hepatitis B virus ( HBV) replication and expression in primary rat hepatocytes ( PRH) transfected with circular viral DNA for further study on the interaction of HBV with hepatocytes.

目的建立乙型肝炎病毒(HBV)环化裸DNA转染原代大鼠肝细胞瞬时表达模型,为进一步研究肝细胞与HBV之间的互动关系奠定基础。

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14、

A gcl conserved sequence of 319 bp, which contained BTB/ POZ domain coding sequence, was isolated from Quail ( Coturnix coturnix) genome DNA using the BTB degenerated primers.

本研究应用简并PCR克隆技术,在鹌鹑(Coturnix coturnix)中扩增得到319bp的gcl基因(包含BTB/POZ结构域编码序列)保守序列。

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15、

A compact capillary electrophoresis ( CE) system based on liquid-core waveguide ( LCW) technique was developed for deoxyribonucleic acid ( DNA) separations with laser induced detection.

报道了一种基于液芯波导的小型激光诱导荧光微流控分析系统及其在分离检测DNA样品中的应用。

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16、

It comes as researchers told a new conference in Texas that they have proof Big foot is real and have even collected DNA samples.

在德克萨斯的一次会议上,研究者表示已经有证据表明大脚怪确实存在,并且他们已经采集到了一些大脚怪的DNA样本了。

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17、

The correlation between various DNA ploid and metastasis of NLN and prognosis were analysed.

将不同DNA倍体的NPC、颈淋巴结转移和预后进行相关性分析。

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18、

The Correlation Between DNA Ploid and Metastasis of NLN and Prognosis in NPC

鼻咽癌细胞核DNA倍体和颈淋巴结转移与预后的相关性

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19、

Inhibition of Bcl2 to DNA damage repair induced by NNK

Bcl2对NNK诱导的DNA损伤后修复的抑制作用

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20、

All the infected animals were dead and viral DNA could be detected from these experimentally infected koi tissues, suggesting that the KHV has prevalent in China.

用组织匀浆上清人工感染健康锦鲤,并从试验感染鱼组织中检测到KHV病毒DNA.该结果表明KHV或CNGV已经传播到我国,应引起重视。

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