1、

The purity of MSCs could be ascertained with the positive identification of double criteria of CD44, or CD54 and Brdu, and negative identification of CD34 and CD45 coloration.

再以CD44、CD54和Brdu双标作为MSCs阳性鉴定,CD34、CD45染色作为MSCs阴性鉴定以确定MSCs的纯度。

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2、

Results The growth and the proliferation velocity of MSCs were active and fast respectively when the pH level of culture medium were between 7.2~ 7.4, MSCs formed the cell clone having the anagogic cell shape and exhibited a typical fibroblast-like morphology.

结果培养液的pH值在7.2~7.4之间时,MSCs生长活跃,增殖速度极快,无接触抑制,镜下细胞呈单一梭形外观的成纤维细胞,形成成纤维细胞集落。

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3、

Methods Human cord blood was drawn out, then MSCs was separated and cultivated.

方法抽取人类脐带静脉血, 进行细胞分离和培养,并进行荧光激活细胞分析.

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4、

The protect function of MSCs to DA is considered to be relevant with the cell factor that MSCs secrete, which is the one of the mechanism of reducing neuronal apoptosis.

MSCs对DA能神经元的保护作用考虑与其能分泌的细胞因子有关,可能是MSCs减少神经元凋亡的机制之一。

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