1、

Methods Anti-ActR ⅱ A C-terminal polyclonal antibody was prepared by immunizing the rabbit with ActR ⅱ A C-terminal protein. The expression of ActR ⅱ A on immunocytes was observed by immunocytochemistry staining.

方法以ActRⅡAC末端肽作为抗原免疫家兔制备特异性抗ActRⅡAC末端抗体,通过免疫细胞化学染色技术观察ActRⅡA在免疫细胞上的分布。

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3、

Results The immunocytochemistry staining of specific Anti-ActR ⅱ A C polyclonal antibody showed that ActR ⅱ A was highly expressed on murine macrophages, but not other immunocytes, such as T, B, NK, and DC cells.

结果采用特异性抗ActRⅡAC末端抗体进行免疫细胞化学染色,结果显示ActRⅡA在小鼠巨噬细胞上高水平表达,而在其它免疫细胞(T、B、NK、DC)上未见表达。

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4、

By mammalian cell two-hybrid, its ability to combine with ActR ⅱ A was confirmed.

采用哺乳细胞双杂交进一步确定ActRIP4与ActRⅡA的相互作用;

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