PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。
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Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.
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经过酶切鉴定和目的片段PCR检测鉴定,证明表达载体构建成功。
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Identify the specificity of PCR products by DNA sequencing and restriction endonuclease reactions
以DNA测序及限制性酶切反应鉴定PCR产物特异性
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Restriction Endonuclease Analysis of Mitochondrial DNA of German Saddle Horse
德国骑乘马线粒体DNA的限制性酶分析
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传统的检测限制性内切酶活性的方法是不连续的,费时的。
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RT-PCR结合限制性内切酶分析法区分NDV强弱毒株
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The study on restriction endonuclease enzyme analysis method in atypical pneumonia
传染性非典型肺炎限制性内切酶检测方法的研究
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After a restriction endonuclease cleavage, two expected smaller fragments were observed.
经限制性内切酶反应,获得两个预期大小的片段。
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Standardization of restriction endonuclease expression in molecular biology books and periodicals
分子生物学书刊中限制性内切酶的规范表达
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Study on restriction endonuclease map of mtDNA of three species in Cyprinidae
三种鱼mtDNA的限制性内切酶分析
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Methods Cell culture and methylation sensitive restriction endonuclease digestion were used.
方法采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。
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豫西地区减蛋综合征病毒基因组酶切图谱分析
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