1、

Restrictive endonuclease site Eag I was eliminated.

突变后Eag内切酶位点消失.

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2、

Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

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3、

Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.

方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.

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4、

Restriction endonuclease and target fragment PCR detection results show that the expression vector was constructed successfully.

经过酶切鉴定和目的片段PCR检测鉴定,证明表达载体构建成功。

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5、

Studies on the Active Groups of Pst I Restriction Endonuclease

限制性核酸内切酶PstⅠ活性基团的研究

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6、

Identify the specificity of PCR products by DNA sequencing and restriction endonuclease reactions

以DNA测序及限制性酶切反应鉴定PCR产物特异性

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7、

Design and expression of an artificial restriction endonuclease

一种人工限制性内切酶的设计与表达

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8、

Restriction Endonuclease Analysis of Mitochondrial DNA of German Saddle Horse

德国骑乘马线粒体DNA的限制性酶分析

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9、

Traditional methods to assay the activity of restriction endonuclease are discontinuous and time consuming.

传统的检测限制性内切酶活性的方法是不连续的,费时的。

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10、

Detection and Typing of Newcastle Disease Virus by RT-PCR Coupled with Restriction Endonuclease Analysis

RT-PCR结合限制性内切酶分析法区分NDV强弱毒株

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11、

The study on restriction endonuclease enzyme analysis method in atypical pneumonia

传染性非典型肺炎限制性内切酶检测方法的研究

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12、

Study on A Type ⅱ Restriction Endonuclease Ncr ⅰ

Ⅱ型限制性核酸内切酶NcrⅠ的研究

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13、

Restriction endonuclease analysis of egg drop syndrome virus

鸡源和鸭源减蛋综合征病毒DNA酶切图谱分析

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14、

After a restriction endonuclease cleavage, two expected smaller fragments were observed.

经限制性内切酶反应,获得两个预期大小的片段。

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15、

Standardization of restriction endonuclease expression in molecular biology books and periodicals

分子生物学书刊中限制性内切酶的规范表达

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16、

Study on restriction endonuclease map of mtDNA of three species in Cyprinidae

三种鱼mtDNA的限制性内切酶分析

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17、

Methods Cell culture and methylation sensitive restriction endonuclease digestion were used.

方法采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。

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18、

Restriction Endonuclease Analysis of Egg Drop Syndrome Virus Isolated from the West Region of Henan Province

豫西地区减蛋综合征病毒基因组酶切图谱分析

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19、

AFM Studies of Interaction of DNA and Restriction Endonuclease

DNA与限制性内切酶相互作用的AFM研究

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20、

Isolation and Restriction Endonuclease Analysis of Rice Chloroplast DNA

水稻叶绿体DNA的分离纯化及其限制酶切分析

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