The insert cDNAs were clonedinto EcoR I-digested, BAP-treated pUC 18 vectors and transformed into E.
同时以EcoR酶消化环状pUC18DNA, BAP对载体5端去磷酸化.
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With use of Mse I-EcoR I digest and primers, fingerprints of the 187 samples were obtained.
利用MseI-EcoR的酶切及引物组合, 建立了梅花及其近缘种187份样品的品种(种)的DNA指纹图 谱.
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