1、

Method: Stimulated the endothelial cells with aqueous areca nut extract ( AANE) and the cultured medium was aspirated. Then the level of NO produced by endothelial cells was detected by method of ELISA.

方法:用槟榔提取物干预体外培养的内皮细胞,收集培养上清,用ELISA方法检测内皮细胞分泌一氧化氮(NO)的浓度。

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2、

The purpose of this study was to establish the animal model of oral submucous fibrosis ( OSF) in buccal mucosa of SD rats by submucosa injecting and surface painting aqueous areca nut extracts ( AANE).

采用粘膜下注射及表面涂布水溶性槟榔提取液(AANE)相结合方法,诱发SD大鼠颊部口腔粘膜下纤维性变(OSF)。

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3、

Objective: To explore the pathogenesis of oral submucous fibrosis ( OSF) by observing the effect of Aqueous areca nut extract ( AANE) on the level of tumor necrosis factor α( TNF α) produced by oral keratinocytes.

目的:观察槟榔提取物对口腔黏膜角朊细胞分泌肿瘤坏死因子α的影响,探讨槟榔诱发口腔黏膜下纤维性变的机制。

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4、

Result: The level of NO produced by endothelial cells was inhibited by AANE.

结果:槟榔提取物能抑制体外培养的内皮细胞分泌NO。

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5、

Result: The concentration of TNF α increased after keratinocytes were stimulated by AANE for 12 hours or longer time.

结果:槟榔提取物干预角朊细胞12h后肿瘤坏死因子α的量开始增加;

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6、

Method: Human oral mucosa keratinocytes were isolated, cultured, stimulated with AANE, The supernatant was aspirated and the level of TNF α was detected using radioimmunoassay.

方法:通过体外培养口腔黏膜角朊细胞,用不同浓度槟榔提取物对其进行干预,放射免疫方法检测不同干预时段培养上清中的肿瘤坏死因子α浓度。

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7、

The effection of mast cells ( MC) on collagen metabolism of oral submucous fibrosis ( OSF) induced by aqueous areca nut extracts ( AANE) in rats was studied by transmission electron microscope, histochemical and biochemical analyses.

借助组织化学、生化分析和透射电镜方法,探讨肥大细胞对水溶性槟榔提取液诱发的大鼠口腔粘膜下纤维性变(OSF)的胶原代谢的影响。

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