1、

Expression of TGF � � 1 mRNA was evaluated by RT PCR.

RT?PCR方法测定肺组织内TGF?β1的表达.

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2、

Results The structures of recombinant plasmids were comfinaed by PCR and sequencing.

结果重组体经PCR和测序证实准确连接.

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3、

REP-PCR , AFLP technique can reveal the genetic differentiation properly.

AFLP和 REP -PCR 技术均能很好地反映菌株的遗传学差异.

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4、

Methods Polymerase chain reaction ( PCR ) connected with reverse dot blot ( RDB ) were performed.

方法采用多聚酶链反应 ( PCR ) 结合反向斑点杂交 ( RDB ) 技术.

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5、

PCR were using to detect bla ( TEM ), bla ( SHV ) and bla ( CTX-M ) genes.

应用聚合酶链反应(PCR)方法扩增产ESBLs株的bla ( TEM ) 、 bla ( SHV ) 和bla ( CTXM ) 基因.

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6、

The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method.

因此,我们利用接头PCR技术克隆了PNZIP基因的启动子.

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7、

RT-PCR was used to determine GK and PEPCKgene expression ( corrected by � �-actin ) .

用RT -PCR 检测GK和 PEPCK mRNA表达水平的变化.

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8、

Histamine-producing strains could be detected by PCR test or DNA probe technology.

利用PCR与DNA探针技术能够快速检测葡萄酒中的组胺产生菌.

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9、

Methods PCR was performed to amplify the AMG encoding region.

方法 采用PCR技术体外扩增AMG完整分泌肽编码区.

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10、

Methods: Polymer-ase chain reaction ( PCR ) was used to determine the ACE genotype.

方法: 采用聚合酶链反应 ( PCR ) 方法检测ACE基因型.

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11、

RT-PCR was used to detect the eNOS mRNA and iNOS mRNA.

RT -PCR 分别检测内皮细胞eNOS、iNOS基因的相对表达量.

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12、

EST-PCR and EST-SSR marker were developed in Chinese cabbage.

建立了白菜的EST -PCR 和EST-SSR标记.

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13、

The mutation was confirmed by allele specific PCR ( ASPCR ).

应用等位基因特异性 PCR 验证测序所发现的突变.

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14、

The expressions of bcl-x, bax and eNOS were measured by SQ-RT-PCR technique.

采用半定量逆转录聚合酶链反应(SQ-RT-PCR)法检测内皮细胞bcl-x 、 bax以及 eNOS表达.

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15、

Results CnA α, CnA � � could be detected by RT-PCR but no CnA � �.

结果RT -PCR 可检测到CnAα 、 CnAβ,未检测到CnAγ.

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16、

PPAR � � expression of GH 3, MMQ and ATt 20 cell line was evaluated by RT-PCR and Western Blot.

RT -PCR 及WesternBlot方法检测PPARγ在GH3 、 MMQ、ATt20等垂体瘤细胞株中的表达情况.

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17、

Expression of TGF � � 1 was evaluated by RT PCR technique.

逆转录聚合酶链反应(RT?PCR)方法测定肺组织内TGF?β1的表达.

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18、

PUC 18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably.

SR-15 菌株通过质粒PUC-18转化和ERIC -PCR 再分离实验验证,结果显示重组菌株在植株体内稳定定位,具有稳定的内生特性.

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19、

Methods Aggrecan IGD cDNA was amplified by RT-PCR and inserted into pUCm-T vector .

方法 取人软骨细胞,逆转录多聚酶链反应(RTPCR)扩增aggrecan球 间区域 ( IGD )片段.

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20、

Methods: Under a condition of heat stress, amplify HSP 70 gene from colon cancer cells by PCR.

方法在热应激条件下, 用PCR法从结肠腺癌细胞获取HSP70基因.

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