pcr的例句大全

Commonly methods of testing include GUS activity detection, PCR technology, hybridization, and immunological detection.

常用检测方法有GUS活性检测, PCR技术, 杂交检测, 免疫学检测.

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RT-PCR was employed to measure the HIF-1α and VEGF mRNA expressions in gastrocnemii.

采用RT -PCR 法检测,半定量分析大鼠腓肠肌HIF -1 αmRNA和VEGFmRNA表达.

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RT-PCR was employed to measure the HIF-1α and VEGF mRNA expressions in gastrocnemii.

采用RT -PCR 法检测,半定量分析大鼠腓肠肌HIF -1 αmRNA和VEGFmRNA表达.

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Methods: Under a condition of heat stress, amplify HSP 70 gene from colon cancer cells by PCR.

方法在热应激条件下, 用PCR法从结肠腺癌细胞获取HSP70基因.

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Methods: Under a condition of heat stress, amplify HSP 70 gene from colon cancer cells by PCR.

方法在热应激条件下, 用PCR法从结肠腺癌细胞获取HSP70基因.

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Hematopoiesis regulation factors such as SCF, IL-3 , TNF-α and IFN-� � mRNA semi-quantitative expression were observed by RT-PCR .

通过RT -PCR 法观察其骨髓造血细胞调控因子干细胞因子(SCF)、白细胞介素3(IL -3 ) 、 肿瘤坏死因子α(TNF - α)、干扰素γ(IFN - γ)mRNA的 半定量 表达情况.

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Methods Aggrecan IGD cDNA was amplified by RT-PCR and inserted into pUCm-T vector .

方法　取人软骨细胞,逆转录多聚酶链反应(RTPCR)扩增aggrecan球 间区域 ( IGD )片段.

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PPAR � � expression of GH 3, MMQ and ATt 20 cell line was evaluated by RT-PCR and Western Blot.

RT -PCR 及WesternBlot方法检测PPARγ在GH3 、 MMQ、ATt20等垂体瘤细胞株中的表达情况.

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PUC 18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably.

SR-15 菌株通过质粒PUC-18转化和ERIC -PCR 再分离实验验证,结果显示重组菌株在植株体内稳定定位,具有稳定的内生特性.

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Expression of TGF � � 1 was evaluated by RT PCR technique.

逆转录聚合酶链反应(RT?PCR)方法测定肺组织内TGF?β1的表达.

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A Study on PCR to Detect Streptomycin and Kanamycin Resistant Gene aadA and aadB of Swine Salmonella

PCR技术检测猪源沙门氏菌链霉素、卡那霉素耐药基因（aadA、aadB）的研究

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Four microsatellite markers linked to FecB locus on 6 chromosome in ovine were analyzed for 108 animals by PCR and denaturing polyacrylamide gel electrophoresis.

选择4个位于绵羊6号染色体上且与FecB基因紧密连锁的微卫星标记，对小尾寒羊基因组进行PCR扩增后，采用最小二乘法估计各等位基因片段对产羔数的影响。

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Semi quantitative RT-PCR analysis show that there were notable difference between different lines. Expression of gene driven by CatB promoter in root is more than in leaf. Expression level of the latter was extremely weak.

半定量RT-PCR结果显示，不同株系间的表达量有明显差异，而且由水稻CatB启动子驱动的外源基因在根中的表达量远远大于在叶子中的表达量，后者只有微弱的表达。

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By using RT-PCR, a study was carried out on the time and spacial order of the diapause hormone gene expression in oriental tobacco budworm on the transcription level.

利用RT PCR法，在转录水平上对烟夜蛾滞育激素（diapause hormone，DH）基因的时空表达进行了研究。

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Cholesterol ester transfer protein ( CETP) gene polymorphism was assayed by PCR-RFLP.

以PCR RFLP检测胆固醇酯转运蛋白（CETP）基因TagⅠB多态性。

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In this study, the 28-day-old broilers from Exp.1 were used as the test materials after slaughtering, and the same segment of jejunum was removed from each chick ( four chicks per treatment) and was prepared for the BBMV and real time PCR.

本试验以试验一中肉仔鸡为试验材料，28日龄时，每个重复取4只鸡，屠宰后取每只鸡相同部位空肠肠段，制备BBMV及用作实时定量检测。

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Results: A total of 4 alleles with 5~ 8 repeat units were observed and the genetic diversity was estimated to be 0.501 3. Decomposed and keratinized samples were typed successfully by using the methods of Chelex-100 with DTT and PCR buffer, respectively.

结果河南汉族214例无关个体中检出4种等位基因，重复5~8次，基因差异度0.5013。腐败肌肉及角化组织用DTT联合Chelex-100法和PCR缓冲液法均分型成功。

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The detection rate of the three techniques ( Pathogenicity test, DIA and PCR assay) for Xac reach 100% for symptomatic citrus sample.

PCR、DIA和致病性测定法检测田间显症样品的检出率均可达到100%，而检测柑桔无症样品的检出率依次降低。

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Weight gain ratio ( WGR), special growth rate ( SGR), feed efficiency ( FE) and, protein conversion efficiency ( PCR) of fish were improved by the elevation of dietary phosphorus level and reached maximal value at 1.32% total phosphorus.

随着饲料中磷添加水平的提高，黑鲷幼鱼的增重率、特定生长率、饲料效率和蛋白质效率呈上升趋势，当饲料总磷含量为1.32%时，各项指标均达到最高水平。

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The full-length of THRSP α gene of ducks cloned by the author was used to detect the polymorphism in THRSP α intron. The association of mutation locus with growth and body compositions of 238 Pekin ducks at 6 wks was investigated by PCR-RFLP method.

根据克隆出的鸭THRSPα基因全长序列，采用PCR-RFLP法检测THRSPα内含子多态与238只6周龄北京鸭生长、屠体性状的关系。

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