1、

To optimize the multiplex RT-PCR, the concentration of primers and annealing temperature were repeatedly tried. Final results showed that a multiplex RT-PCR system to simultaneously detect HLB, CEVd and CTV was firstly established successfully.

就影响多重PCR的主要因素引物浓度和退火温度进行了一系列优化,建立了能同时检测HLB、CEVd、CTV的多重RT-PCR技术体系。

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2、

The results showed the primers have the specificity of Capripoxvirus and do not have consensual reaction with the Orf virus of parapoxvirus, Fowl pox virus of Avipoxvirus and the skin samples of healthy goats.

研究结果表明两对引物具有羊痘病毒属特异性,不与副痘病毒属羊传染性脓疱病毒、禽痘病毒属鸡痘病毒、健康山羊皮肤样本发生交叉反应。

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3、

The electrophoresis results of RAPD showed that 12 primers produced 96 bands with molecular weight ranged 350~ 2 000 bp and the shared bands was 17 with polymorphism rate of 82.29%. This showed the rich genetic diversity of the Chinese Shar-pei in its origin place.

筛选出的12个重复性、多态性好的随机引物,扩增出96个位点,分子量在350~2000bp,共享带数为17条,多态性82.29%,说明沙皮犬存在丰富的遗传多样性。

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4、

Detection of the genotypes of human platelet alloantigen 2,3,5 by PCR sequence specific primers

PCR-SSP检测血小板同种抗原2、3、5系统基因型

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5、

Degenerate Primers Polymerase Chain Reaction Clone and Identification of the Acetylcholinesterase Gene Fragment from the Mosquito, Aedes albopictus; Detection of Unknown Virus from 2 Case of Aseptic Meningitis Epidemic Using Random Primer PCR Assay

白纹伊蚊乙酰胆碱酯酶基因片段简并引物PCR、克隆及鉴定随机引物聚合酶链反应法鉴定2例无菌性脑炎的未知病毒

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6、

A trademark used for a transparent thermoplastic acrylic resin employed in paints, enamels and primers.

卢塞特树脂一种透明热塑料丙烯酸树脂的一个商标,这种树脂用于油漆 、 搪瓷、雷管中.

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7、

By designing primers in the conservative coding sequences flanking a polymorphic site, it is possible to exploit a PCR-based marker called amplified consensus genetic marker ( ACGM), which is transferable among different species.

通过在多态位点两侧的保守编码序列上设计引物,可以开发出在不同物种间通用的基于PCR的分子标记,称为扩增共有序列遗传标记(ACGM)。

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8、

A gcl conserved sequence of 319 bp, which contained BTB/ POZ domain coding sequence, was isolated from Quail ( Coturnix coturnix) genome DNA using the BTB degenerated primers.

本研究应用简并PCR克隆技术,在鹌鹑(Coturnix coturnix)中扩增得到319bp的gcl基因(包含BTB/POZ结构域编码序列)保守序列。

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9、

Two pair of primers containing attB adapter were designed separately by Gateway cloning technology. Platinum pfx DNA polymerase which can reduce the non-specific binding greatly was used during two PCR in which adding B sequence to the cloned gene.

根据Gateway克隆技术的要求,设计含有attB接头的引物,利用高保真的PlatinumpfxDNA聚合酶,通过PCR方法在克隆基因的两端加上B序列。

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10、

One of two positive clones was sequenced and analyzed by Peculiar primers directIy. The results of sequencing and restriction analysis indicated that the positive clone contains the part of human alpha-lactalbumin gene. The physical mny of about 10 kb fragment of the positive clone was made.

特异引物直接对粘粒阳性克隆进行部分序列测定及鉴定分析,结果进一步证明该阳性克隆含有不完全的人α-乳清白蛋白基因,并绘出了该阳性克隆约10kb的片段物理图谱。

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11、

Preliminary study on molecular systematics of bamboo by SSR primers derived from Rice

用水稻微卫星引物进行竹子分子系统学研究初探

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12、

We designed four pairs gene primers of hla, hlb, hlg, hld for PCR identification and studied the distribution the hemolysin genes in Staphylococcus aureus isolates.

本试验设计合成hla、hlb、hlg、hld四对金黄色葡萄球菌溶血素基因引物,进行PCR鉴定,并对溶血素基因在金葡菌分离株中的分布状况进行研究。

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13、

RT-PCR method was established to identify the virus by type 1 and novel DHV primers respectively.

利用Ⅰ型和新型鸭肝炎病毒鉴别引物对提取的病毒RNA进行了RT-PCR扩增。

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14、

Compatible with any shop primer, epoxy or epoxy-tar primers.

底漆可以是各种车间底漆 、 环氧类漆或环氧煤沥青类漆.

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15、

BRV was detected by RT-LAMP using the primers, and the reaction conditions were optimized.

用此引物对提取好的病毒RNA进行RT-LAMP反应,并对试验反应条件进行优化。

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16、

We chose 10 random primers, which is suitable to amplify the 16 kinds of mulberry materials, and analyze the result by RAPD.

选用重复性较好的10个随机引物对保存于新疆的16份桑种质材料进行了RAPD分析,共获得139个RAPD标记。

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17、

Methods: According to specific sequence of Treponema pallidum gene ( tpp47 gene), a pair of specific primers were designed and PCR assay was developed.

方法:根据梅毒螺旋体47ku膜蛋白基因序列,自行设计筛选一对特异寡核苷酸引物,建立梅毒螺旋体的PCR。

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18、

A trademark used a transparent thermoplastic acrylic resin employed in paints , enamels, and primers.

一种不饱和液态羧基酸, 用于制造丙烯酸树脂.

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19、

We can't use these designed primers as primers designed on the mutated-type site of Pinb-D 1 b .

这几种突变体的筛选上,不能像Pinb -D1b 突变体一样根据突变位点设计引物使用.

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20、

The results showed that among 60 primers of OPG, OPH and OPK series, 21 primers could amplify the polymorphic bands, which proved that RAPD provided a lot of genetic markers for population genetic analysis of rice blast fungus.

60个引物中有21个扩增出多态性条带,表明福建省稻瘟菌群体有丰富的RAPD多态性,可为该菌的遗传分析提供大量的遗传标记。

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