TLH

Objective To prepare both polyclonal antibodies of anti-Vibrio Parahaemolyticus and anti-thermolabile hemolysin ( TLH) for establishing the enzyme-linked immunosorbent assay ( ELISA) double antibodies sandwich method to detect TLH.

目的分别制备抗副溶血弧菌及其不耐热溶血毒素（thermolabile hemolysin，TLH）的多克隆抗体，建立检测副溶血弧菌TLH的酶联免疫吸附试验（ELISA）双抗体夹心法。

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Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.

方法利用PCR技术扩增tlh基因，克隆至载体pET32a+并测序。将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。

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Cloning and sequence analysis of the tlh gene of vibrio parahaemolyticus

副溶血弧菌tlh基因的克隆及序列分析

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The shorter operation time and less average amount of blood loss were observed in TVH group than that in the LAVH and TLH/ LH groups.

TVH组平均手术时间及平均出血量均明显低于LAVH和TLH组（P0.001）。

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Objective To compare the clinical efficacy between total laparoscopic hysterectomy ( TLH) and total abdominal hysterectomy ( TAH).

目的比较腹腔镜全子宫切除术（total laparoscopic hysterectomy，TLH）与开腹全子宫切除术的临床效果。

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Conclusion Compared with LAVH, TLH is feasible to deal with bigger uterus with less blood loss and shorter surgery duration and without more frequent complications.

结论TLH组切除的子宫体积较大，不影响出血量和手术时间，且并发症较少。

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The result shows that TLH and enriching blood decoction of angelica can improve the suppressing effect of CY to rat model, but the variation of the remedy are different on immunity regulation.

结果表明，不同配伍组均能改善免疫功能低下小鼠的免疫功能，但又各有侧重。

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Conclusions: TLH is a safe and efficient method for some diseases if it is used properly.

结论：TLH在一定条件下是治疗某些疾病安全、有效的方法。

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The result showed that A, B and TLH could increase the ratio of lymphocyte transformation ( P0.05), and B was the best one in the three groups.

结果显示：①A方、B方与TLH组均能提高淋巴细胞转化率（P＜0.05），B方最佳。

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Molecular cloning, expression and functions of the tlh gene of Vibrio parahaemolyticus

副溶血弧菌tlh基因的克隆、表达及功能的研究

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Objective To compare the clinical outcomes of total abdominal hysterectomy ( TAH), vaginal hysterectomy ( VH) and total laparoscopic hysterectomy ( TLH) in short term.

目的对比分析开腹子宫切除术（TAH）、经阴道子宫切除术（VH）和腹腔镜下全子宫切除术（TLH）的短期临床效果。

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ObjectiveTo express and obtain the fused protein thermolabile hemolysin ( TLH) of vibrio parahaemolyticus ( VP).

目的表达和纯化融合表达的副溶血弧菌不耐热溶血毒素。

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