ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA.

Conclusions Treponema pallidum induced mainly the expression of CD11c~ (+) DC and initiated cell-mediated immune response.

结论梅毒螺旋体（TP）进入体内后主要诱导CD11c+型DC表达，引发Th1反应，启动细胞免疫；

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Objective To find out the distribution of subtypes of T. pallidum ( TP) in Hengyang area and to provide laboratory basis for molecular epidemiological studies of TP infection.

目的了解衡阳地区梅毒螺旋体（TP）的分子亚型的分布，为梅毒螺旋体感染分子流行病学研究提供实验依据。

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Objective To study the relationship between the different courses of Treponema pallidum ( TP) infection and the immunological changes of the host.

目的探讨梅毒螺旋体（TP）感染的不同病程与宿主免疫学变化的关系。

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Diagnosis of Neonatal Congenital Syphilis with Treponema pallidum IgM Western Blot

梅毒螺旋体IgM抗体蛋白印迹试验诊断新生儿胎传梅毒的探讨

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Furthermore, isolation and purification processes compromise the structural integrity of the fragile organisms.

另外，因梅毒螺旋体十分脆弱，分离和纯化过程可破坏Tp结构的完整性。

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Application value of FQ-PCR for detection of treponema pallidum DNA

荧光PCR技术检测梅毒螺旋体DNA的意义

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Objective To evaluate an enzyme-linked immunosorbent assay ( ELISA) technique based on recombinant antigens for detection of antibodies against Treponema pallidum.

目的评价基因重组抗原ELISA法在梅毒螺旋体抗体检测中的意义。

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The 4 rapid tests and TPHA were performed by using whole blood and serum respectively.

分别取全血和血清进行梅毒螺旋体血凝试验（TPHA）及4种试剂盒的检测。

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Objective To approach double-primer PCR assay for simultaneous detection of Herpes simplex virus ⅱ( HSV-2) and Treponema pallidum.

目的建立双引物聚合酶链反应（PCR）用于同时快速检测梅毒螺旋体和单纯疱疹病毒Ⅱ型。

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Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity, and hence can be applied in the diagnosis of syphilis in various periods.

结论梅毒螺旋体特异性IgM抗体检测灵敏度高、特异性强，适用于未经治疗的各个时期的梅毒诊断。

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Clinical evaluation of specific IgM treponema pallidum antibody detection

梅毒螺旋体特异性IgM抗体检测的临床评价

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An optimized method has been established to simultaneously detect multiple antibodies of HIV, TP and HCV in the serum by the way of microarray.

建立一种用蛋白质微阵列法可同时检测血清中艾滋病毒（HIV），丙型肝炎病毒（HCV）和梅毒螺旋体（TP）抗体的方法。

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51 clinical samples were detected by dark-field microscopy and a double-primer PCR, the positive rates were 15.7% and 19.6%, respectively.

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

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Diagnostic Significance of IgM Antibodies to Treponema Pallidum in Cerebrospinal Fluid for Neurosyphilis

梅毒螺旋体IgM抗体对于神经梅毒的诊断意义

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Controlled experiments of testing anti-TP and anti-HIV by DIGFA and EIA

免疫胶体金与酶免疫检测HIV抗体和梅毒螺旋体抗体对照实验

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Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods.

目的探讨梅毒螺旋体特异性IgM抗体检测在各个时期梅毒诊断中的意义。

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Methods 2557 cases were detected by using ELISA with double antigen sandwich before blood transfusion.

方法：采用免疫ELISA法检测2557例输血前患者血清梅毒螺旋体抗体。

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Objective: To establish PCR assay for rapid detection of Treponema pallidum.

目的：建立聚合酶链反应（PCR）用于快速检测梅毒螺旋体。

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Conclusions In view of its high specificity and sensitivity, TP-IgM-WB could be used as a confirmatory test for the diagnosis of both symptomatic and asymptomatic neonatal congenital syphilis.

结论梅毒螺旋体IgM抗体蛋白印迹试验可作为一种确诊试验应用于新生儿胎传梅毒，尤其是胎传潜伏梅毒的早期诊断。

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Methods: According to specific sequence of Treponema pallidum gene ( tpp47 gene), a pair of specific primers were designed and PCR assay was developed.

方法：根据梅毒螺旋体47ku膜蛋白基因序列，自行设计筛选一对特异寡核苷酸引物，建立梅毒螺旋体的PCR。

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