1、

Studies on Cultivar Classification of Osmanthus fragrans by ISSR-PCR Analysis

ISSR-PCR技术在桂花品种分类研究中的应用

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2、

The shoot meristems were transformed. But no plants were positive, according to PCR results. 6.

对茎尖进行了转化,只获得抗性植株,但未检测到PCR阳性植株。

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3、

Expression of TGF � � 1 was evaluated by RT PCR technique.

逆转录聚合酶链反应(RT?PCR)方法测定肺组织内TGF?β1的表达.

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4、

PUC 18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably.

SR-15 菌株通过质粒PUC-18转化和ERIC -PCR 再分离实验验证,结果显示重组菌株在植株体内稳定定位,具有稳定的内生特性.

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5、

PPAR � � expression of GH 3, MMQ and ATt 20 cell line was evaluated by RT-PCR and Western Blot.

RT -PCR 及WesternBlot方法检测PPARγ在GH3 、 MMQ、ATt20等垂体瘤细胞株中的表达情况.

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6、

Methods Aggrecan IGD cDNA was amplified by RT-PCR and inserted into pUCm-T vector .

方法 取人软骨细胞,逆转录多聚酶链反应(RTPCR)扩增aggrecan球 间区域 ( IGD )片段.

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7、

Hematopoiesis regulation factors such as SCF, IL-3 , TNF-α and IFN-� � mRNA semi-quantitative expression were observed by RT-PCR .

通过RT -PCR 法观察其骨髓造血细胞调控因子干细胞因子(SCF)、白细胞介素3(IL -3 ) 、 肿瘤坏死因子α(TNF - α)、干扰素γ(IFN - γ)mRNA的 半定量 表达情况.

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8、

Methods: Under a condition of heat stress, amplify HSP 70 gene from colon cancer cells by PCR.

方法在热应激条件下, 用PCR法从结肠腺癌细胞获取HSP70基因.

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9、

Methods: Under a condition of heat stress, amplify HSP 70 gene from colon cancer cells by PCR.

方法在热应激条件下, 用PCR法从结肠腺癌细胞获取HSP70基因.

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10、

RT-PCR was employed to measure the HIF-1α and VEGF mRNA expressions in gastrocnemii.

采用RT -PCR 法检测,半定量分析大鼠腓肠肌HIF -1 αmRNA和VEGFmRNA表达.

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11、

RT-PCR was employed to measure the HIF-1α and VEGF mRNA expressions in gastrocnemii.

采用RT -PCR 法检测,半定量分析大鼠腓肠肌HIF -1 αmRNA和VEGFmRNA表达.

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12、

Commonly methods of testing include GUS activity detection, PCR technology, hybridization, and immunological detection.

常用检测方法有GUS活性检测, PCR技术, 杂交检测, 免疫学检测.

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13、

Commonly methods of testing include GUS activity detection, PCR technology, hybridization, and immunological detection.

常用检测方法有GUS活性检测, PCR技术, 杂交检测, 免疫学检测.

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14、

METHODS � �-Lactamase genes including TEM, SHV, OXA-10 , PER, VEB, GES, CARB, IMP, VIM, SPM, GIM, DHA, FOX, MOX and oprD 2 were detected by PCR amplification in 33 PAE isolates.

方法对33株铜绿假单胞菌,采用PCR检测β - 内酰胺类药物耐药相关基因(TEM 、 SHV 、 OXA -10 群 、 PER 、 VEB 、 GES 、 CARB 、 IMP 、 VIM 、 SPM 、 GIM 、 DHA 、 FOX 、 MOX和OprD2).

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15、

Thrombopoietin ( TPO) cDNA obtained by RT-PCR from human fetal liver

利用RT-PCR从人胎肝获得血小板生成素cDNA

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16、

Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.

方法利用PCR技术扩增tlh基因,克隆至载体pET32a+并测序。将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。

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17、

Detection and Identification of Food Borne Pathogens Using Real Time PCR and Gene Chip Assay

运用实时荧光PCR和DNA芯片技术检测和鉴定食源性致病菌方法的研究

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18、

Chitinase gene had been amplified by PCR in albinos lethal mutant line, that certificated transformation was successful;

PCR扩增结果表明在白化致死突变株系中存在几丁质酶基因,证明转化成功;

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19、

One step RT-PCR is practisible for ARV RNA detection.

表明该一步法RT_PCR对于ARV的检测是可行的.

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20、

Methods: PCR technique was used to determine the ACE gene genotypes in 102 cases of Chinese type ⅱ diabetics.

方法:用PCR技术检测102例中国汉族Ⅱ型糖尿病患者ACE基因I/D多态基因型。

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