1、

Conclusion : FQ-PCR is absolutely necessary methods for diagnosis CMV infection in pregnant women.

结论FQ -PCR 对CMV感染的诊断有重要的作用.

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2、

The restriction digestion system consisted of 10μ L PCR products, 2.0μ L 10× Buffer, 83.350 nkat restriction enzyme and 7.5μ L ddH 2 O.

PCR产物10.0μL, 加入2.0μL10×Buffer, 83.350nkat内切酶,7.5μL双蒸水构成20μL酶 切反应体系.

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3、

RT-PCR kit was obtained from Takara Company.

RT-PCR试剂盒为hkaa产品,引物均由北京奥科公司合成。

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4、

Genome DNA was extracted by TKM method. Exon 10 of TSHR gene sequence was amplified, PCR production was detected by direct and reverse sequencing, and the results and human TSHR gene sequence Genbank were compared.

用TKM法提取基因组DNA,PCR扩增TSHR基因第10外显子序列,对PCR产物正反向测序,测序结果同Genbank人TSHR基因序列对比。

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5、

The full length CMO cDNA was cloned from Salicornia europaea by RT-PCR and RLM-RACE.

本研究利用RT -PCR 和RLM-RACE技术获得了盐角草的CMOcDNA全序列.

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6、

After PCR and sequencing, the expression plasmid pWD κ H was transfected into CHO ( dhfr ~-) cells.

经PCR和测序鉴定正确后, 用lipofectAMINE2 000转染CHO ( dhfr - ) 细胞.

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7、

D 13 S 317 locus was sequencing by Beckman CEQ 8800. The PCR products were analyzed by PAG vertical electrophoresis.

用BeckmanCEQ 8800毛细管测序仪测定序列, 应用PCR技术和聚丙烯酰胺凝胶垂直板电泳分型.

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8、

Objective : To establish fluorescence quantitative nested RT-PCR method for detecting Borna disease virus ( BDV ).

目的: 建立博尔纳病病毒 ( BDV ) 荧光定量套式RT -PCR 检测方法,为快速、准确地定量检测BDV奠定基础.

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9、

Analysis of PCR-RFLP on LPL Gene of Six Chicken Breeds

6个品种鸡LPL基因的PCR-RFLP分析

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10、

The P1 structure gene was amplified by PCR method.

采用PCR扩增方法获取P1结构基因。

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11、

Screening Genes Related to Piglet ETEC F4 Receptor with δ DDRT-PCR Method

δ差异显示法筛选仔猪ETEC F4受体相关基因

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12、

RT-PCR for Diagnosis of Rubella and Analysis on E1 Region DNA Sequencing

RT-PCR诊断风疹及E1膜蛋白基因序列分析

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13、

The PCR and hybridization results were compared.

将唐氏综合征患者的荧光定量PCR检测结果和荧光原位杂交检测结果与细胞遗传学分析结果进行比较。

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14、

The results showed that the typical expressions of GVHD were observed 4 or 5 days after the injection of donor spleen cells, and specific male Y chromosome fragment was amplified by genomic PCR in female BALC/ c receipts.

结果显示:3注射脾细胞4~5天后,小鼠开始出现GVHD典型体征,第10天受体小鼠体内扩增出雄性供体小鼠Y染色体特异片段。

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15、

The library was screened by using 20 microsatellite markers and 5 known functional genes with PCR. Positive BACs for all these markers and genes were identified successfully and the positive number ranges from 3 to 19 with average of 11.3.

用20个微卫星标记和5个功能基因对整个文库进行PCR筛选,所有这些标记和基因都得到了阳性克隆,克隆数从3到19不等,得到的平均阳性克隆数为11.3。

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16、

C 3 d-P 28 coding gene was amplified by PCR.

将PCR获得 的补体C3d-P28DNA序列 以头尾串连的方式构建四聚体.

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17、

PCR founded in 1985, as a amplification technology in vitro, it acts in life science study importantly in recent ten years.

PCR是1985年兴起的一项基因体浙江大学硕土学位论文外扩槽的分于生物学新技术,多年来在生命科学研究领域发挥了前所未有的作用。

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18、

Application of real-time fluorescence quantitative PCR accompanied with comparison of Delta CT for diagnosis of Down's syndrome from a single cell

单细胞实时荧光定量PCR结合比较阈值法在诊断唐氏综合征中的应用

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19、

The relationship between genital tract cytomegalovirus infection and tubal pregnancy DETECTION OF HUMAN CYTOMEGALOVIRUS BY PCR

输卵管妊娠患者生殖道巨细胞病毒检测与基因分析人巨细胞病毒的PCR检测

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20、

Method The genotypes and alleles of D4 receptor gene in 104 refractory schizophrenic patients and 76 healthy controls were examined with PCR amplification technique.

方法用PCR技术测定104例难治性精神分裂症病人和76例正常人的D4受体基因。

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