plasmid的例句大全

Conclusion: pED GCSF is a high eukaryotic expression plasmid in mammalian cell.

结论: pED-GCSF是一个能在哺乳动物细胞中高效表达rhG-CSF的真核表达质粒.

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The relationship between plant mitochondrial plasmid DNAs and cytoplasmic male sterility ( CMS ) is discussed.

最后,综述了植物线粒体DNA质 粒与植物的细胞质雄性不育 ( CMS ) 之间的关系.

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Objective To control the quality of mouse nerve growth factor ( NGF ) DNA plasmid for gene therapy.

目的对小鼠神经生长因子 ( NGF ) 基因治疗型DNA质粒进行质量控制.

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Method The recombinant plasmid pMD 18-T-gG was constructed as HSV-2 DNA standard for quantitative analysis.

方法构建重组质粒pMD18-T-gG作为标准品.

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The recombinant plasmid pPGVT 3 derived from the vector pUC 4 was unstable in the E.

从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃ 培养得不到转化子.

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Objective To construct the GST fusion protein expression plasmid of cardiotrophin-1 ( CT-1 ) and tetanus toxin C frag-( ment ( TTC ). )

目的构建心肌营养素 -1 ( CT -1 ) 和破伤风 外毒素 C片段 ( TTC ) 重组融合蛋白表达质粒.

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Objective To evaluate effect of ultrasound contrast agent on expression of plasmid green fluorescent protein ( GFP) in tibialis anterior muscle of Wistar rats.

目的评价诊断超声照射下微泡造影剂对绿荧光蛋白（green fluorescent protein，GFP）质粒在Wistar大鼠胫前肌中表达的作用。

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Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.

方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.

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Objective To investigate the amelogenin expression of recombinant plasmid PcDNA 3-AMG in COS-1 cell line.

目的研究人釉原蛋白(AMG) 重组质粒PcDNA3-AMG在COS -1 细胞系中的表达.

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In this review, we summarize the current progresses on plasmid biology in the rare actinomycetes.

本文综述目前稀有放线菌中的质粒生物学的研究进展.

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The traditional alkaline method to isolate plasmid DNA takes andsystem requires strict control.

实验室中常用的质粒提取方法是碱裂解法.

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Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay.

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；

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[ Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability.

方法使用替代试剂，建立改良的碱裂解法进行质粒DNA提取，并用琼脂糖电泳验证所得的质粒的质量，紫外测量估算产量及纯度。限制性酶切验证其用度。

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The function of the isolated strain DSP was located on a plasmid, and the possibility of using using the degradative plasmid to enhance chlorpyrifos degradation in soil was investigated.

同时对毒死蜱降解菌的降解功能定位及其降解质粒pDOC在土壤中的应用进行研究。

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Methods: Four oligonucleotide probes were designed and labeled for plasmid Gap-LCR-ELISA, which were derived from CT cryptic plasmid. Then these probes were used to detect six standard serotype strains of CT and other bacteria causing the infection of genitourinary tract in clinic.

方法：根据CT共有隐蔽性质粒设计并标记4条寡核苷酸探针，对6型CT标准株和鹦鹉热衣原体及其他常见泌尿生殖道病原菌行Gap-LCR-ELISA。

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Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

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It's a cryptic plasmid. This has provided more basic information for further study of the N.

该质粒的功能有待进一步研究。为进一步研究利用N。

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Objective Plasmid of AAV vector with HIV 1 gag and gp 120 were constructed respectively.

目的构建带有HIV1gag或gp120基因的重组腺病毒伴随病毒AAVHIVgag或AAVHIVgp120.

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The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.

重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。

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A Modified Method for Isolation of Plasmid DNA from E.coli

大肠杆菌质粒DNA提取方法的优化

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