plasmid的例句大全

Construction of HNP-3 Mature Peptide Bait Plasmid of Yeast Two-hybrid System and Detecting Its Self-activating and Toxic Effect

防御素HNP-3成熟肽酵母双杂交诱饵质粒的构建及自激活和毒性检测

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REMI transformation efficiency was 19.12~ 22.72 times higher than normal plasmid transformation without restriction enzyme.

实验结果还表明，REMI的转化效率是一般质粒转化效率的19.12～22.72倍。

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ResuIts: The result of restriction fragment analysis tallied with the structural map of hTR plasmid vector.

结果：酶切鉴定的结果与hTR质粒图谱吻合。

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Construction of recombinant HVT transferring plasmid vector with fusion gene cassette of NDV F_ ( 48) E_ 9 strain

新城疫病毒F（48）E9株病毒F基因表达盒的火鸡疱疹病毒转移质粒载体构建

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Methods: Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates, and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains.

方法：采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性；碱裂解法分析产青霉素酶的淋病奈瑟菌（PPNG）株及耐四环素的淋病奈瑟菌（TRNG）株的质粒图谱；

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The plasmid DNA was extracted by Alkaline Lysis, and separated by electrophoresis on the gel.

Alkaline Lysis小量快速提取质粒DNA，凝胶电泳拍照；

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An improved alkaline lysis adaptive to extracting plasmid DNA of Bacillus

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

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Results A recombinant plasmid pBV/ IL-2/ PEA was constructed.

结果经鉴定，获得重组质粒pBV／IL－2／PEA。

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Objective To construct a prokaryotic expression plasmid encoding 3 phosphoglycerate kinase ( PGK) gene of Clonorchis sinensis and analyze its expression in E.

目的构建编码华支睾吸虫3磷酸甘油酸激酶基因的原核表达载体，并分析其在大肠埃希菌中的表达。

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Methods Construct the plasmid for expressing the C terminal of ActR ⅱ A C, analyze the expressed protein by SDS PAGE, and immunize rabbits with GST ActR ⅱ A C terminal polypeptide to prepare corresponding antibody.

方法构建GST ActRⅡAC末端蛋白表达质粒，经SDS page分析表达蛋白。

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Two bacterial strains, which could degrade pyridine and quinoline seperately were isolated from activated sludge. The purpose of this study was to investigate the degradative characteristics and plasmid characteristics of the two bacterial strains.

本文的研究目的是对从活性污泥中筛选的两株分别降解吡啶和喹啉的高效降解菌进行了生理生化特性及降解特性的研究，并考察两株降解菌上赋存质粒的特性。

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Studies on isolation of 1,2,4-trichlorobenzene-degrading strain and its degradative plasmid

1,2,4-三氯苯降解菌的分离及其降解质粒的研究

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Experimental results showed that contact hemolysis of Shigella spp and EIEC was correlated with the plasmid encoded-invasive protein antigens.

实验结果表明，接触性溶血试验与志贺氏菌及EIEC大质粒编码的“侵袭相关蛋白”的表达具有相关性。

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The induction of RNR 3 gene was investigated in S . cerevisiae using RNR 3-LacZ fusion plasmid.

构建RNR3-LacZ基因融合质粒,用于检测酵母细胞内RNR3基因的诱导性.

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A recombinant plasmid pUC-IL 6 coding for IL 6 was constructed by recombinant gene technique.

利用基因重组技术,构建含IL6基因的重组质粒pUC - IL6.

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After PCR and sequencing, the expression plasmid pWD κ H was transfected into CHO ( dhfr ~-) cells.

经PCR和测序鉴定正确后, 用lipofectAMINE2 000转染CHO ( dhfr - ) 细胞.

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Construction of recombinant plasmid highly expressing luciferase in Drosophila S2 cells

果蝇S2细胞中高表达荧光素酶重组质粒的构建及活性测定

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The plasmid could be expressed by in vitro transfection of BHK cells.

体外转染BHK细胞可检测到重组质粒的转录表达；

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Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

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Methods: Recombinant plasmid DNA of Hendra virus G gene were amplified by conventional PCR and real-time PCR assays, and sensitivity of the two methods was compared.

方法：采用常规PCR法和实时PCR法分别对构建的Hendra病毒全长G基因的重组质粒DNA进行扩增，并比较两种方法的敏感性。

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