1、

METHODS the K-B manner and MIC are used to obtain drug-fast spectrum and the alkaline lysis is to extract small quantity of plasmid is got from cataphoresis of 0.7% agarose congelation.

方法耐药谱选用K&B法和MIC、Alkaline Lysis小量快速提取质粒,0.7%琼脂糖凝胶电泳获得质粒DNA图谱。

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2、

Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

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3、

The plasmid DNA was extracted by Alkaline Lysis, and separated by electrophoresis on the gel.

Alkaline Lysis小量快速提取质粒DNA,凝胶电泳拍照;

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4、

An improved alkaline lysis adaptive to extracting plasmid DNA of Bacillus

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

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5、

Methods: Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates, and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains.

方法:采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性;碱裂解法分析产青霉素酶的淋病奈瑟菌(PPNG)株及耐四环素的淋病奈瑟菌(TRNG)株的质粒图谱;

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Objective To establish the recombinant plasmid of small interfering RNA ( siRNA) against matrix metalloproteinase-26 ( MMP-26), observe dumbness effect of the MMP-26 expression in human carcinoma, and explore the new method of gene therapy.

目的构建基质金属蛋白酶-26(MMP-26)小干扰RNA(siRNA)表达质粒,以研究其对人肿瘤细胞MMP-26基因表达的沉默效果,探索肿瘤基因治疗的新途径。

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The effect of α_1(ⅰ) collagen recombinant antisense expressive plasmid on HLF cells

α1(Ⅰ)型前胶原基因反义重组质粒对成纤维细胞的影响

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8、

The final yield of plasmid DNA from the broth after alkaline lysis is 56.61%, and its purity determined by agarose electrophoresis is almost 100%.

结果表明:以碱裂液中质粒DNA为计算基准的得率为56.61%、超螺旋质粒DNA的电泳纯度100%。

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Our previous study showed that recombinant plasmid carrying with human hepatocyte growth factor ( pHGF) can promote CRW healing, however, the mechanism of promoting CRW healing by pHGF has not completely elucidated.

目的:本实验室前期研究表明,携带人肝细胞生长因子的重组质粒(pHGF)对放创复合伤具有很好的促愈作用,但其促愈机理尚未完全阐明。

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10、

A recombinant PBR322 plasmid with inserts of operator sequence for lambda repressor and Bgl ⅱ site was constructed.

构建了插入λ阻抑蛋白(Rep)的操纵基因(OR)和BglⅡ识别位点的PBR322重组质粒。

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11、

The recombinant plasmid structure was verified by double-enzyme cleavage and gene sequence analysis.

该质粒经双酶切鉴定、目的基因序列分析,证实了重组质粒结构的正确性.

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12、

The Construction and Functional Expression of Bifunctional Plasmid

双功能质粒的构建与功能表达

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The plasmid transforming activity is positive re lated with the frequency of the plasmid DNA's breakage.

质粒转化活性的大小与DNA单双链断裂频率呈正相关.

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14、

Preparation and nature of plasmid in purpl nonsulfur bateria

紫色非硫细菌质粒的制备与性质

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Binary vectors for genetic engineering consist of one plasmid containing the VIR region and a second containing the T-DNA including the foreign DNA.

用于基因工程的双载体系统由一个含有VIR区的质粒和一个含有T-DNA的质粒组成,其中T-DNA含有外源基因。

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16、

Construction and Identification of hGM CSF Expressing Plasmid Which can be Stably Expressed in Mammalian Cells

hGM-CSF基因在哺乳动物细胞中稳定转染表达质粒的构建和鉴定

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17、

Purified ATF-lys 10 specifically bound to uPAR-expressing cells and formed protein-DNA complexes with plasmid pGL 3-control.

将uPAR反义核酸表达质粒与ATF一 lyslo以及多聚赖氨酸组装成基因转移复合物,并转染95D细胞.

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18、

Results The Arresten gene was screening successfully, and the recombinant plasmid pBV 220-Arr was constructed successful.

结果成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达.

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19、

Aim a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

目的:消减文库构建过程中,用PCR技术快速筛选重组阳性克隆.

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20、

Objective To survey the antimicrobial resistance and plasmid profiles of Neisseria gonorrhoeae isolated from a hospital.

目的了解某院淋病奈瑟菌对抗菌药的耐药性及质粒谱型.

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