1、

The total DNAs from 3 species of plants and 2 species of animals were amplified by RAPD ( random amplified polymorphic DNA) using 10 RAPD 10 nt primers and 3 different commercial Taq DNA polymerases, respectively.

随机选用了10条RAPD引物,采用3种商品taq DNA聚合酶体系,以5个不同物种的动植物总DNA为模板,进行RAPD扩增。

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2、

[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated.

【方法】对模板DNA、引物、dNTP mixture、taq DNA聚合酶浓度及退火温度设置不同梯度,研究各因素对PCR结果的影响;

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3、

The graft polymerization processes could be controlled by monomer concentration, UV-irradiation time and photoinitiator concentration.

接枝聚合过程可以通过改变单体浓度、紫外辐照时间以及光引发剂浓度来进行调控。

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4、

Methods Slides of metaphase chromosome were examined by the method of PCR in situ. And PCR reactions without Taq DNA polymerase, primer and bio 11 dUTP were set up as control groups.

方法应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测,并设立PCR反应液中无taq DNA聚合酶、无引物、无bio-11-dUTP等进行多组对照。

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5、

In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系,对影响SSR-PCR扩增的模板DNA浓度、Mg~(2+)的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

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6、

Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

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7、

After Taq DNA polymerase had been isolated and purified from the thermophilic bacteria ( Thermus aquatics) in aquatic habitat by Randall K. Saiki and his colleagues in 1988, it was widely used in PCR techniques.

1988年,Randall K.Saiki等[1.2]从水生栖热菌(Thermus aquatics)中分离纯化到了taq DNA聚合酶,之后它广泛应用于PCR技术。

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8、

The relationships of molecular weight and monomer concentration, initiator concentration show that the polymerization reaction system has the characteristic of living polymerization.

分子量与单体浓度、引发剂浓度的关系说明,该体系具有一定程度的活性聚合特点。

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9、

Although the commercial production of Taq DNA polymerase has come true for many years and a lot of technique improvement has been made, the activity ratio of the enzyme preparation is not high, some methods of procedure too complicated, or the production cost is high.

虽然taq DNA聚合酶已经商品化生产多年,制备方法较多,但是目前一些方法生产的酶制剂比活性不高,而一些方法又过于繁琐,增加生产成本。

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10、

With genome DNA as template, three upstream primers ( R1 、 R2 、 R3) targeting point mutation of K-ras oncogene codon 12 and common downstream primer ( R4) were added respectively. PCR was employed to amplify corresponding fragment with Taq DNA polymerase.

以基因组DNA为模板,分别加入针对K-ras第12密码子的三种主要突变方式设计的PCR上游引物R1、R2、R3和共同的下游引物R4,在taq DNA聚合酶作用下扩增相应片段。

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11、

Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

方法:将环化质粒以限制性内切酶消化成线性后,以Klenow大片段将末端补平,再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

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12、

The other preparation conditions, such as monomer concentration, temperature, and time of polymerization, were systematically studied.

本文还对其它制备条件,比如单体浓度,温度,聚合反应时间都进行了系统研究。

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13、

Kinetics of Vinyl Monomer/ NSubstituted Maleimide Copolymerization

乙烯基单体/N-取代马来酰亚胺共聚合动力学

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14、

A study on graft polymerization of vinyl monomer onto carbon black

乙烯基单体在炭黑表面上的接枝聚合

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15、

Studies on the atom transfer radical copolymerization of vinyl monomer and vinyl monomer containing nitroxide group

乙烯基单体与含氮氧稳定自由基单体的原子转移自由基共聚合研究

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16、

With the increase of monomer concentration, polymer particle size increases.

随着单体浓度的增大,聚合物粒子的粒径随之增大。

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17、

Graft copolymerization of betaine type vinyl monomer onto hydroxyethylcellulose

甜菜碱型烯类单体与羟乙基纤维素的接枝聚合

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18、

The layers of montmorillonite contain Lewis acid sites and transition metal ions, which could be used for catalytic polymerization of vinyl monomer.

蒙脱土片层含有Lewis酸点及过渡金属离子可用于烯类单体的催化聚合反应;

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19、

Propagation Rate Constants for the Cationic Polymerization of Vinyl Monomer

乙烯基单体正离子聚合中链增长速率常数的研究进展

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20、

When the monomer concentration increased, the grafting rate, initial polymerization rate and final conversion rate increased, but the grafting efficiency decreased;

单体浓度上升,接枝率、初始聚合速率和最终转化率也上升,但接枝效率则下降;

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