1、

Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

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2、

In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系,对影响SSR-PCR扩增的模板DNA浓度、Mg~(2+)的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

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3、

Methods Slides of metaphase chromosome were examined by the method of PCR in situ. And PCR reactions without Taq DNA polymerase, primer and bio 11 dUTP were set up as control groups.

方法应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测,并设立PCR反应液中无taq DNA聚合酶、无引物、无bio-11-dUTP等进行多组对照。

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4、

[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated.

【方法】对模板DNA、引物、dNTP mixture、taq DNA聚合酶浓度及退火温度设置不同梯度,研究各因素对PCR结果的影响;

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5、

The total DNAs from 3 species of plants and 2 species of animals were amplified by RAPD ( random amplified polymorphic DNA) using 10 RAPD 10 nt primers and 3 different commercial Taq DNA polymerases, respectively.

随机选用了10条RAPD引物,采用3种商品taq DNA聚合酶体系,以5个不同物种的动植物总DNA为模板,进行RAPD扩增。

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6、

High content of protein, phenol, tannin, pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响taq DNA聚合酶的活性,是导致PCR扩增反应失败的主要因素。

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7、

Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR.

在等位基因特异PCR中,Mg2+、dNTP及taq DNA聚合酶的用量均大于普通PCR。

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8、

Polyvinyl fluoride coatings are based on resin made by the polymerization of a vinylidene fluoride monomer.

氟乙烯树脂涂料是在亚乙烯基氟化物为单体聚合物而成的树脂为基础上制成涂料.

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9、

It could act not only as a radical trapper but an initiator of ATRP.

它不但可以用作自由基聚合的引发剂,同时也可以作为自由基的捕捉剂.

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10、

The waster polymer produced from the process of dry-acrylic fiber has extensive application value.

在干法腈纶生产过程中产生的废聚合物具有广泛的使用价值.

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11、

The product have a soft point 130 ℃ and high valent of use.

产物聚合松香的软化点为130℃,具有较高的应用价值.

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12、

Functions: it contains protein fiber, natural aloe moisturizer, vegetal protective glue, protein gene, purified water.

功效: 特含蛋白纤维 、 天然芦荟保湿剂 、 植物聚合保护胶 、 蛋白质基因 、 纯净水等.

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13、

This invention discloses a kind of way to produce the porous polymer velum.

本发明公开了一种制备多孔聚合物膜的方法.

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14、

Thermally induced phase separation ( TIPS ) procedure is a simple and versatile membrane preparation technique.

热诱导相分离法 ( TIPS ) 是一种简单而有效的制备聚合物微孔膜的方法.

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15、

The videodisc is made of liquid crystal polymers, about the size of a phonograph record.

光盘是由液晶聚合物制成的, 大小和一个唱片差不多.

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16、

The sticky kettle and anti sticking principles of vinyl chloride were analysed.

分析了氯乙烯聚合过程的粘釜和防粘机理.

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17、

Silicones in general are exceptionally stable and inert.

硅酮通常都很稳定,属惰性聚合物.

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18、

Useing unsaturated urethane , 2-ethylhexyl acrylate, crosslinking agent, high oil-absorbent resin was prepared by suspension polymerization.

用不饱和氨基甲酸酯 、 丙烯酸异辛酯和交联剂, 以悬浮聚合的方法制备高吸油性树脂.

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19、

It a wide range of technological applications in catalysts, acting as molecular sieves, molecular ( ion ) exchangers.

该聚合物在选择性催化 、 类分子筛 、 主客体分子 ( 离子 ) 交换等方面有很好的应用前景.

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20、

This paper introduces the application of polyol drilling fluid in Hongtai area of Tuba oilfield.

对聚合醇钻井液在吐哈油田红台地区钻井中的应用情况进行了概述.

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