1、

A silent nucleotide change was introduced into the cDNA clone to create a Xba I site ( C to T at 8453nt) that is used as a genetic marker of this infectious clone. This genetic marker was retained in the genome of recovered progeny virus.

全基因组cDNA克隆构建过程中引入的沉默突变(8453位核苷酸由C变为T)产生Xba I酶切位点作为遗传标记,在子代恢复病毒的基因组中稳定存在。

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2、

Analysis on differential expression profile of cytochrome P450 genes in benzene poisoning cells using cDNA microarray

用cDNA微阵列芯片检测苯中毒细胞色素P450基因表达差异

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Methods: ( 1) Reverse transcription-polymerase chain reaction ( RT-PCR) was use to obtain the TR gene complementary DNA ( cDNA).

方法:(1)进行逆转录-聚合酶链式反应(RT PCR),获得TR基因cDNA;

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4、

Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension

重叠区扩增基因拼接法在构建膜锚定金黄色葡萄球菌肠毒素A融合基因上的应用

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5、

The invasive ability of two ovarian tumor cell lines were compared by Boyden chamber invasion assay before and after VEGF cDNA transfected.

Boyden小室体外侵袭实验比较VEGF cDNA转染2株癌细胞前后通过人工重组基底膜的能力变化。

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