1、
Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.
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2、
Finally, DNA ligase newly synthesized DNA chain and the original links.
最后DNA连接酶将新合成的DNA链与原来的链接上.
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3、
Thus, the NEU-induced primary chromosome breakage may not be repaired by the ligase.
由NEU所诱发的染色体初级断裂不能为连接酶所修复。
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