lysis的例句大全

Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

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Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

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The plasmids were extracted by alkaline lysis technique.

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

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The procedure of Southern hybridization has been improved as follows: the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation.

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

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The final yield of plasmid DNA from the broth after alkaline lysis is 56.61%, and its purity determined by agarose electrophoresis is almost 100%.

结果表明：以碱裂液中质粒DNA为计算基准的得率为56.61%、超螺旋质粒DNA的电泳纯度100%。

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Objective To evaluate the incidence of depress in hemodia lysis and peritoneal dialysis uremia patients.

目的评价进行血液透析和腹膜透析治疗的尿毒症患者抑郁症的发病情况.

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[ Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability.

方法使用替代试剂，建立改良的碱裂解法进行质粒DNA提取，并用琼脂糖电泳验证所得的质粒的质量，紫外测量估算产量及纯度。限制性酶切验证其用度。

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The plasmid DNA was extracted by Alkaline Lysis, and separated by electrophoresis on the gel.

Alkaline Lysis小量快速提取质粒DNA，凝胶电泳拍照；

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In conclusion, sufficient DNA for PCR can be quickly extracted from transgenic rice food by alkaline lysis method. The stability of recombinant DNA in transgenic was different under different processing conditions.

由此可知，碱处理法能够快速、有效的提取转基因稻米制品中DNA，转基因稻米中的各种外源成分在不同加工条件下的稳定性不同。

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An improved alkaline lysis adaptive to extracting plasmid DNA of Bacillus

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

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Methods: Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates, and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains.

方法：采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性；碱裂解法分析产青霉素酶的淋病奈瑟菌（PPNG）株及耐四环素的淋病奈瑟菌（TRNG）株的质粒图谱；

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Samples were extracted with the 80% precooling and acidified methanol and conjugated abscisic acid in extracts were treated by alkaline lysis method.

样品以80%酸化预冷甲醇提取，使用碱裂解法分析检测ABA结合态。

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Conclusion The alkaline lysis method is simple, rapid and efficient for extraction of serum HBV-DNA and suitable for HBV-DNA quantitative PCR detection.

结论碱裂解法提取HBV-DNA简单、快速，适用于HBV-DNA荧光定量测定。

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Pycnosis in most mitochondrion, swelling and cristae lysis in come mitochondrion, more shrinkage bandelette formated in myofibril, hyperemia and edema in interstitial and more vacuoles in the endothelial cell of blood capillary were observed in diabetes with cerebral ischemia group.

糖尿病加脑缺血组树鼩可见：大部分线粒体固缩，部分线粒体肿胀、嵴溶解，心肌纤维较多收缩带形成，间质充血水肿，毛细血管内皮细胞见较多空泡。

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To evaluate the effectiveness and safety of hysteroscopic lysis for treatment of severe intrauterine adhesions ( IUA) due to endometrial tuberculosis.

目的探讨宫腔镜治疗由子宫内膜结核引起的重度宫腔粘连（IUA）的安全性和有效性。

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LZM in combination with CFT blocked the lysis of bacteria without disturbance of bactericidal effect of CFT.

ZM与CFT联合使用既能阻止细菌溶解，又能保持与CFT单独用药相当的杀菌作用；

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C5b-9_(( n)), as an multieffect immune factor, can insert into cell membrane making cell lysis. Recently, the new researches demonstrates that non-lethal dose MAC can deposit on many

C5b-9（（n））是CNS炎症过程中一种多效免疫因子，在补体大量活化后，可插入细胞膜导致细胞溶解死亡，而近年来新的研究发现，非致死剂量的MAC可沉积于中

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Lysis rate was influenced by the voltage and duration of the pulse and the conductance of the cell suspension.

胞解率与脉冲宽度、电压和细胞悬浮液的电导率都有关。

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Observations on the Efficacy of Needle Knife Lysis in Treating Genual Arthropathy due to Rheumatoid Arthritis

针刀松解术治疗类风湿关节炎引起膝关节病变疗效观察

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