聚合的例句大全

Optimization of Recombinant Taq DNA Polymerase Expression in E.coli

重组taq DNA聚合酶在大肠杆菌中表达条件的优化

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Study on the Functional Domain of Taq DNA Polymerase

taq DNA聚合酶功能区域的定位

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Optimization of Preparation Techniques of Taq DNA Polymerase

taq DNA聚合酶制备技术的优化

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Taq DNA polymerase is an ideal enzyme for DNA sequencing because it's fast, highly processive and active over a broad range of temperature.

taq DNA聚合酶具有反应速度快、温度作用范围广及良好的续进性等特点，可视为一种理想的DNA顺序分析酶。

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Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR.

在等位基因特异PCR中，Mg2+、dNTP及taq DNA聚合酶的用量均大于普通PCR。

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High content of protein, phenol, tannin, pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响taq DNA聚合酶的活性，是导致PCR扩增反应失败的主要因素。

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The total DNAs from 3 species of plants and 2 species of animals were amplified by RAPD ( random amplified polymorphic DNA) using 10 RAPD 10 nt primers and 3 different commercial Taq DNA polymerases, respectively.

随机选用了10条RAPD引物，采用3种商品taq DNA聚合酶体系，以5个不同物种的动植物总DNA为模板，进行RAPD扩增。

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[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated.

【方法】对模板DNA、引物、dNTP mixture、taq DNA聚合酶浓度及退火温度设置不同梯度，研究各因素对PCR结果的影响；

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Methods Slides of metaphase chromosome were examined by the method of PCR in situ. And PCR reactions without Taq DNA polymerase, primer and bio 11 dUTP were set up as control groups.

方法应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测，并设立PCR反应液中无taq DNA聚合酶、无引物、无bio-11-dUTP等进行多组对照。

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In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系，对影响SSR-PCR扩增的模板DNA浓度、Mg~（2+）的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

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Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

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After Taq DNA polymerase had been isolated and purified from the thermophilic bacteria ( Thermus aquatics) in aquatic habitat by Randall K. Saiki and his colleagues in 1988, it was widely used in PCR techniques.

1988年，Randall K.Saiki等[1.2]从水生栖热菌（Thermus aquatics）中分离纯化到了taq DNA聚合酶，之后它广泛应用于PCR技术。

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Although the commercial production of Taq DNA polymerase has come true for many years and a lot of technique improvement has been made, the activity ratio of the enzyme preparation is not high, some methods of procedure too complicated, or the production cost is high.

虽然taq DNA聚合酶已经商品化生产多年，制备方法较多，但是目前一些方法生产的酶制剂比活性不高，而一些方法又过于繁琐，增加生产成本。

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With genome DNA as template, three upstream primers ( R1 、 R2 、 R3) targeting point mutation of K-ras oncogene codon 12 and common downstream primer ( R4) were added respectively. PCR was employed to amplify corresponding fragment with Taq DNA polymerase.

以基因组DNA为模板，分别加入针对K-ras第12密码子的三种主要突变方式设计的PCR上游引物R1、R2、R3和共同的下游引物R4，在taq DNA聚合酶作用下扩增相应片段。

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Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

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This component has one function, and it is called by the feed aggregator at the very beginning of the program invocation.

此组件有一个函数，在程序调用的最早阶段由提要聚合器调用。

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The community NGOs, who can aggregate interests and self-govern, provides a kind of organization path for the growth of civism.

而社区民间组织所具有的利益聚合和自治参与功能，为社区成员公民精神的成长提供了组织化的空间。

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Study on the Polymerization of Acrylamide Initiated by Bifunctional Initiator

双官能度引发剂引发丙烯酰胺聚合研究

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Atom Transfer Radical Polymerization of Methyl Methacrylate Using Bifunctional Initiator, 4-( Chloromethyl) Phenyltrimethoxysilane

4-（氯甲基）苯基三甲氧基硅烷引发的甲基丙烯酸甲酯的原子转移自由基聚合

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The tacticity of polymers is mainly influenced by the polarity of substituent group of vinyl monomer.

作者认为影响聚合物等规度的因素主要是取代基的极性效应。

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