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In vitro culture system of buffalo fetal fibroblasts ( BFF ) was investigated in this study.
本实验探讨了适合水牛胎儿成纤维细胞 ( BFF ) 体外培养的体系.
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Therefore adding 10 % of mixed BFF in maturation medium is the best choice.
因此,成熟培养液中添加10%的混合BFF较为适宜.
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Among the three groups adding BFF at 10 % concentrationexpressed the best proliferous effect.
细胞的表型,以添加10%BFF的培养液促增殖效果最好.
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Therefore, adding 10 % of mixing BFF in maturation medium could be the best choice.
因此, 成熟培养液中以添加10%的混合BFF较为适宜.
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对比 10%bFF 和10%胎牛血清(FBS),以及观察微滴培养对卵母细胞成熟的影响.
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Along with the increase of bFF, in IVF group , cleavage and blastocyst rate decreases.
添加D0血清体外受精胚胎卵裂率较高, 但对囊胚发育率影响差异不显著(P>0.05).
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In addition , the addition of 20 % bFF in maturation medium inhibited the hatching of blastocyst.
添加20%卵泡液的培养液对囊胚的孵化有抑制作用.
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BFF的这种受精抑制因子可能来自血清而不是卵泡细胞.
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