pcr的例句大全

? Methods The total RNA extracted from normal human breast tissue was processed by RT-PCR to produce hLF cDNA.

方法从人乳腺组织中提取出的总RNA进行逆转录，生成cDNA，以其为模板进行PCR扩增人乳铁蛋白cDNA。

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Method Nested RT-PCR method was used to detect PML/ RARa fusion gene.

方法应用筑巢式逆转录聚合酶链反应（RT-PCR）检测PML/RARa融合基因。

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Materials and methods: Placentae and deciduas were obtained from 20 women in the following groups: 10 preterm labor samples, 10 preterm samples from artificial labor. Expressions of the 18 chemokine receptors were accomplished by RT-PCR.

材料与方法：选择复旦大学妇产科医院分娩的早产及对照组病人各10例，在娩出后即采集胎盘组织及蜕膜组织标本，用RT－PCR法检测18种趋化因子受体的表达；

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DNA extraction from different part of oil sunflower mature seeds for PCR analysis

向日葵种子不同部位微量提取DNA用于PCR的研究

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Conclusion The alkaline lysis method is simple, rapid and efficient for extraction of serum HBV-DNA and suitable for HBV-DNA quantitative PCR detection.

结论碱裂解法提取HBV-DNA简单、快速，适用于HBV-DNA荧光定量测定。

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In conclusion, sufficient DNA for PCR can be quickly extracted from transgenic rice food by alkaline lysis method. The stability of recombinant DNA in transgenic was different under different processing conditions.

由此可知，碱处理法能够快速、有效的提取转基因稻米制品中DNA，转基因稻米中的各种外源成分在不同加工条件下的稳定性不同。

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Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification

碱裂解法提取重组质粒DNA及PCR验证

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Detection of abundance of Pseudomonas in environmental samples by real-time quantitative PCR

实时荧光定量PCR法检测环境假单胞菌属细菌丰度

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51 clinical samples were detected by dark-field microscopy and a double-primer PCR, the positive rates were 15.7% and 19.6%, respectively.

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

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Development and Initial Application of nested RT-PCR Method for Japanese Encephalitis virus

日本脑炎病毒套式RT-PCR方法的建立与初步应用

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Detection of Epstein-Barr virus DNA by two temperature PCR cycles

双温聚合酶链反应检测EB病毒

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Amplification of the DNA Polymerase I Gene of Treponema Pallidum by PCR and its Application in Diagnosis of Primary Syphilis

PCR扩增梅毒螺旋体poIA基因及其在一期梅毒诊断中的应用

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Methods: According to specific sequence of Treponema pallidum gene ( tpp47 gene), a pair of specific primers were designed and PCR assay was developed.

方法：根据梅毒螺旋体47ku膜蛋白基因序列，自行设计筛选一对特异寡核苷酸引物，建立梅毒螺旋体的PCR。

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Objective: To establish PCR assay for rapid detection of Treponema pallidum.

目的：建立聚合酶链反应（PCR）用于快速检测梅毒螺旋体。

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Methods: Amniocentesis and detect syphilis DNA by PCR were carried out on 8 pregnant syphilis patients;

方法：对8例不同孕周患梅毒的孕妇抽取羊水用PCR方法检测梅毒螺旋体DNA；

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Isolation of Microsatellite Markers in Eriobotrya japonica Using 5 ′ Anchored PCR

利用5′锚定PCR技术分离枇杷微卫星标记

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Establishment of fluorescent PCR Method for Detection of SARS coronavirus and the clinical trial

荧光聚合酶链反应检测严重急性呼吸综合征冠状病毒的方法建立及临床初步应用

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Methods: RT-PCR was used to examine the expression of Notch-1 and its ligands on cord blood cells.

方法：RT PCR用于检测Notch1及其配体在脐带血细胞上的表达情况。

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Application value of FQ-PCR for detection of treponema pallidum DNA

荧光PCR技术检测梅毒螺旋体DNA的意义

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Objective To approach double-primer PCR assay for simultaneous detection of Herpes simplex virus ⅱ( HSV-2) and Treponema pallidum.

目的建立双引物聚合酶链反应（PCR）用于同时快速检测梅毒螺旋体和单纯疱疹病毒Ⅱ型。

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